大鼠生前与死后损伤肌肉组织中自噬相关蛋白表达  

Expression of Autophagy-Associated Protein in Rat Muscle Tissues after Antemortem and Postmortem Injury

作　　者：时福霞 李鹏[1] 鲁翰霖 李娜[1] 杜秋香[1] 王英元[1] 孙俊红[1] SHI Fu-xia;LI Peng;LU Han-lin;LI Na;DU Qiu-xiang;WANG Ying-yuan;SUN Jun-hong(School of Forensic Medicine,Shanxi Medical University,Jinzhong 030600,Shanxi Province,China)

机构地区：[1]山西医科大学法医学院,山西晋中030600

出　　处：《法医学杂志》2020年第3期293-298,共6页Journal of Forensic Medicine

基　　金：国家自然科学基金面上基金资助项目(81571852);山西省高等学校大学生创新创业训练计划资助项目(2019191)。

摘　　要：目的研究大鼠骨骼肌损伤后BECN1、LC3和p62三种自噬相关蛋白的表达,探讨其在鉴别生前与死后损伤中的应用。方法 72只健康成年Sprague-Dawley大鼠被随机分为未损伤的对照组、生前损伤组(0.5、1、2、4、8、16和24 h)和死后损伤组(0.5、1、2和4 h),制作大鼠右后肢损伤模型。运用Western印迹法检测对照组、生前损伤组以及死后损伤组骨骼肌中自噬蛋白BECN1、LC3-2/LC3-1和p62的表达,分别将数据中心化和标准化处理,建立生前损伤和死后损伤正交偏最小二乘判别分析(orthogonal partial least square-discriminate analysis,OPLS-DA)鉴别模型。结果大鼠骨骼肌挫伤后BECN1、p62蛋白的表达以及LC3-2/LC3-1值在生前损伤和死后损伤组中虽有不同程度的变化,但差异无统计学意义。BECN1的表达量及LC3-2/LC3-1值经中心化和标准化处理后,可区分生前损伤和死后损伤。生前损伤和死后损伤OPLSDA模型提取的主成分对模型的解释程度较好(Rx^2=0.563,Ry^2=0.439),但预测程度稍差(Q^2=0.366)。结论大鼠骨骼肌损伤局部组织中BECN1、LC3-2/LC3-1值可用于生前损伤和死后损伤的鉴别。Objective To study the expression of the three autophagy-associated proteins,BECN1,LC3 and p62,after the injury of the skeletal muscle of rats and to explore its application in differentiation between antemortem and postmortem injury.Methods The 72 healthy Sprague-Dawley rats were randomly divided into the undamaged control group,the antemortem injury group(0.5 h,1 h,2 h,4 h,8 h,16 h and 24 h)and postmortem injury group(0.5 h,1 h,2 h and 4 h).A model of the injured right h2 i/nLdC 3 li-m1 b aonfd r apts62,w aisn ctohnes trcuocntterdo.l Tghreo uepx,p rtehses ioannste omfo rttheem a uitnojuprhya gygr-oausspo cainatde dp opsrtotmeoirntse,mB EinCjuNr1 y,gLrCo3 u-p were detected by Western blotting method.The data were respectively centralized and standardized and the orthogonal partial least square-discrimination analysis(OPLS-DA)identification model of antemortem and postmortem injury groups was constructed.Results The expression of BECN1,p62 protein and LC3-2/LC3-1 after the injury of the skeletal muscle of the rats showed different degrees of changes,but the differences among the 3 groups had no statistical significance.Antemortem and postmortem injury groups can be distinguished by centralizing and standardizing the expression levels of autophagy protein BECN1 and the ratio of LC3-2/LC3-1.The principal components extracted from OPLS-DA model of antemortem injury and postmortem injury had a relatively good interpretation of the model(Rx^2=0.563,Ry^2=0.439),but it were less predictive(Q^2=0.366).Conclusion The expression of BECN1 and the ratio of LC3-2/LC3-1 in injured local tissue of the rat skeletal muscle can be used for the differentiation of antemortem injury group and postmortem injury group.

关 键 词：法医病理学 创伤和损伤 自噬相关蛋白 骨骼肌 生前损伤 死后损伤 大鼠 

分 类 号：D919[医药卫生—法医学]