分散聚合制备功能性单分散水凝胶微球及粒径调控  

Preparation of Functional Monodisperse Hydrogel Beads by Dispersion Polymerization and Regulation of Particle Size

作　　者：任翊 陈钰 马宏[1] Ren Yi;Chen Yu;Ma Hong(School of Life Science,Beijing Institute of Technology,Beijing,100081)

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《生命科学仪器》2020年第3期66-71,共6页Life Science Instruments

基　　金：国家自然科学基金项目(U1532264)。

摘　　要：国内外学者主要聚焦无交联剂存在下分散聚合研究,而交联剂存在的分散聚合研究报道很少。本文以分散聚合方法,在交联剂存在下,制备具有功能基团的刚性水凝胶微球。通过光学显微镜、扫描电镜、核磁、动态光散射等分析方法确认水凝胶微球呈现单分散性,粒径分布在1-2μm。动态光散射粒径研究表明微球粒径随聚乙二醇(Polyethylene glycol,PEG)分子量增长而显著变小,通过调整丙烯酸酯-PEG-生物素的PEG链长可有效调控微球粒径。其中,生物素-PEG2000-微球(以下简称PEG2000微球)平均粒径为1.3μm,微球的尺寸非常适合在经链霉亲和素修饰的1.5μm芯片微坑内反应并固载。微球功能基团经过点击化学反应,可有效负载荧光修饰的寡核苷酸。在荧光显微镜下,微球显现均匀的荧光亮度,且保持单分散性。相关成果可用于核酸快速检测芯片开发等领域,在医学检测方面有很好的应用前景。The study of dispersion polymerization had been mainly conducted in the absence of cross-linking agent,and few reports on the study of dispersion polymerization in the presence of cross-linking agent.In this paper,rigid hydrogel beads with functional groups were prepared by dispersion polymerization method in the presence of a crosslinking agent.It was confirmed by optical microscopy,scanning electron microscopy,nuclear magnetic resonance,dynamic light scattering and other analytical methods that the hydrogel beads exhibited monodispersity and the particle size distribution was 1-2μm.In the study of particle size through dynamic light scattering,it is found that the particle size of the beads diminished with the increase of molecular weight of PEG,that is,adjusting the length of the PEG chain can effectively regulate the particle size of the beads.BiotinPEG2000-beads,with average particle size of 1.3μm,was chose as suitable candidate fastened in the 1.5μm chip pit modified by Streptavidin.After Click reaction of functional groups on beads,fluorescent modified oligonucleotides can be effectively attached into hydrogel beads.Under the fluorescence microscope,the microspheres showed uniform fluorescence brightness and kept monodispersity.The related results could be used in fields such as rapid nucleic acid detection chip development,with favorable application prospects in medical detection.

关 键 词：分散聚合 水凝胶微球 粒径 动态光散射 单分散性 

分 类 号：O631[理学—高分子化学]