骨内H型血管在去势骨质疏松症模型中的变化  

Changes of type H vessel in bone from osteoporotic mice induced ovariectomy

作　　者：王亮[1] 盛茂[2] 袁晔[3] 王爱飞[3] 杨挺伟 赵宇[1] 李平元 周飞[5] 王磊[1] 徐又佳[3] Wang Liang;Sheng Mao;Yuan Ye;Wang Aifei;Yang Tingwei;Zhao Yu;Li Pingyuan;Zhou Fei;Wang Lei;Xu Youjia(Department of Orthopaedics,The Affiliated Suzhou Science&Technology Town Hospital,Nanjing Medical University,Suzhou 215123,China;Department of Radiology,The Affiliated Childrens'Hospital,Soochow University,Suzhou 215025,China;Department of Orthopaedics,Yixing City Shanjuan Orthopaedic Hospital,Wuxi 214000,China;Department of Orthopaedics,The Second Affiliated Hospital,Soochow University,Suzhou 215000,China;Cambridge-Suda Genomic Resource Center,Soochow University,Suzhou 215006,China)

机构地区：[1]南京医科大学附属苏州科技城医院骨科,215123 [2]苏州大学附属儿童医院影像科,215025 [3]苏州大学附属第二医院骨科,215000 [4]善卷骨科医院骨科,无锡214000 [5]苏州大学剑桥-苏大基因组资源中心,215006

出　　处：《中华骨科杂志》2020年第13期873-879,共7页Chinese Journal of Orthopaedics

基　　金：国家自然科学基金(81773439/81874018/81600380);苏州市科技计划项目(SS201814/SS201746);苏州市虎丘区医疗卫生科技计划项目(2018Q006/2016Z010/2019Q014)。

摘　　要：目的通过建立并观察骨质疏松小鼠模型中骨内H型血管(CD31/EMCN染色双阳性)的表现,探讨骨密度与骨内H型血管的变化关系。方法选取雌性8周龄野生型C57BL/6J小鼠30只,分为假手术组、切除卵巢组(ovariectomy,OVX组),每组15只。假手术组手术中仅暴露双侧卵巢,并切除其周围少许脂肪组织;OVX组手术中完整切除双侧卵巢。4周后取材股骨标本以无菌纱布包裹暂存于生理盐水中,以备Micro CT扫描观察骨密度及骨小梁显微结构的变化。取材胫骨标本并剔除其表面软组织,经固定、脱钙、脱水及包埋等处理,进行免疫荧光染色观察两组小鼠骨标本中H型血管的变化,检测两组数据并行统计学分析。结果经Micro CT扫描,OVX组骨密度为(0.11±0.01)g/cm3,假手术组为(0.21±0.01)g/cm3,差异有统计学意义(P=0.001)。骨小梁显微结构发生变化:OVX组骨小梁体积分数为11.52%±1.77%,假手术组为25.87%±1.31%,差异有统计学意义(P<0.05);OVX组骨小梁数目为(1.67±0.33)个/mm,假手术组为(2.95±0.82)/mm,差异无统计学意义(P=0.07);OVX组骨小梁厚度为(0.06±0.01)mm,假手术组为(0.07±0.01)mm,差异有统计学意义(P=0.021)。OVX组骨小梁间隙为(0.29±0.15)mm,假手术组为(0.19±0.01)mm,差异有统计学意义(P<0.05)。除两组间骨小梁数目无统计学意义以外,其余两组骨小梁参数间的差异存在统计学意义(P<0.05)。两组小鼠的骨标本经免疫荧光染色的标准流程处理后,分别计算两组骨标本同一部位H型血管的显像面积,OVX组H型血管的面积占比为9.14%±0.99%,而假手术组H型血管面积占比为29.33%±1.22%,OVX小鼠胫骨干骺端H型血管较假手术组显著减少,两组间差异存在显著的统计学意义(P<0.05)。结论在切除小鼠双侧卵巢模拟绝经后骨质疏松症的模型中,骨密度及骨小梁显微结构发生变化的同时也存在骨内H型血管的改变,这提示H型血管可能参与了绝经后骨质疏松症的发�Objective To explore the changes of bone mineral density (BMD) and type H vessel, which was recently identified as strongly positive for CD31 and Endomucin (CD31hiEmcnhi) in long bone from ovariectomized (OVX) mice compared withSham group.Methods C57BL/6Jwild-type mice were used for experiments and bone tissuewas collected. Eight-week-old female mice were randomly divided into bilateral ovariectomy (OVX) and a sham operation (Sham). The bilateral ovaries were exposed and removed in the OVX group. In the sham group, the ovaries were only exposed but left intact. After 4weeks, these mice were killed for experiment and the femurs were collected for Micro CT scanning in order to observe the changes of bone mineral density (BMD) and trabecular indexes, including bone volume (BV), total volume of interest (TV), bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N). The fresh tibia of each mouse was fixed, decalcified, dehydrated and embedded for immunostaining. All experimental data were analyzed with t-test.Results Mouse femora from two groups were dissected at 4 week time points, and the attached soft tissue was completely removed for Micro CT scanning. BMD in OVX is 0.11±0.01 g/cm3 and 0.21±0.01 g/cm3 in Sham, which indicated the BMD in OVX significantly decreased. The results showed significant difference between the groups (P=0.001). The microarchitecture in trabecular bone changed. BV/TV in OVX is 11.52%±1.77% and 25.87%±1.31% in Sham, which indicated the BV/TV in OVX significantly decreased. The results showed significant difference between the groups (P<0.05). Tb.N in OVX is 1.67±0.33/mm and 2.95±0.82/mm in Sham, which indicated the Tb.N in OVX slightly decreased. The results showed no significant difference between the groups (P=0.066). Tb.Th in OVX is 0.06±0.01 mm and 0.07±0.01 mm in Sham, which indicated the Tb.Th in OVX significantly thinned. The results showed significant difference between the groups (P=0.021). Tb.Sp in OVX is 0.29±0.1

关 键 词：骨质疏松 卵巢切除术 骨密度 

分 类 号：R580[医药卫生—内分泌] R-332[医药卫生—内科学]