薰衣草精油对睡眠剥夺大鼠行为学及血清中胃饥饿素、瘦素含量的影响  

Effects of lavender essential oil on the behavior of sleep-deprived rats and the content of ghrelin and leptin in serum

作　　者：于姣姣 郭理想[2] 戴宁[1] 李杰[1] 何青鋆 刘燕[1] 于雪[1] 吴凤芝[1] 李峰[1] Yu Jiaojiao;Guo Lixiang;Dai Ning;Li Jie;He Qingyun;Liu Yan;Yu Xue;Wu Fengzhi;Li Feng(Beijing University of Chinese Medicine,Beijing 100029;Karamay Central Hospital of Xinjiang)

机构地区：[1]北京中医药大学,北京100029 [2]新疆克拉玛依市中心医院

出　　处：《现代中医临床》2020年第3期49-55,共7页Modern Chinese Clinical Medicine

基　　金：国家自然科学基金面上项目(No.81874428);北京中医药大学新奥奖励基金(No.2018XAJLJJ-023)。

摘　　要：目的探讨薰衣草精油对睡眠剥夺大鼠行为学及血清中胃饥饿素、瘦素含量的影响。方法32只雄性Wistar大鼠随机分为空白组、模型组、薄荷组和薰衣草组。采用改良多平台法进行连续72 h睡眠剥夺,造模结束后,正常组和模型组给予蒸馏水熏蒸,薄荷组和薰衣草组分别给予对应的1%浓度精油熏蒸,每组45 min。治疗结束后,进行抓握力、旷场、高架十字迷宫测试,并测定大鼠血清中胃饥饿素和瘦素含量。结果与空白组相比,模型组大鼠肌张力降低、旷场总活动距离、总穿格数减少、修饰时间增加、直立次数减少、直立时间减少、高架迷宫中开放臂停留时间百分比减少、血清中胃饥饿素含量增加,差异有统计学意义(P均<0.05);与模型组相比,薄荷组大鼠肌张力升高,旷场总活动距离、总穿格数、直立次数、直立时间增加,高架迷宫开放臂进入次数百分比和开放臂停留时间百分比增加、血清中胃饥饿素含量减少,差异有统计学意义(P均<0.05);与模型组相比,薰衣草组大鼠肌张力升高、旷场总活动距离、总穿格数增加、修饰时间减少、直立次数、直立时间增加、高架迷宫开放臂进入次数百分比和开放臂停留时间百分比增加、血清中胃饥饿素含量减少,差异有统计学意义(P均<0.05)。结论薰衣草精油香薰治疗可能是通过调节体内胃饥饿素的含量,达到改善睡眠剥夺大鼠的焦虑情绪并提高活动量的目的。Objective To investigate the effects of lavender essential oil on behavior and serum levels of ghrelin and leptin in sleep-deprived rats.Methods 32 male Wistar rats were randomly assigned to the blank group,model group,mint group and lavender group.After the modeling,the normal group and the model group were fumigated with distilled water,and the mint group and lavender group were fumigated with corresponding 1%concentration of essential oil,respectively,for 45 mins for each group.After the treatment,grip strength,open space and elevated cross maze were tested,and the contents of ghrelin and leptin in the serum of the rats were determined.Results As compared with the blank group,in model group were significantly decreased lowered muscle tone,shortened total distance,less wear lattice,increased modification time,reduced upright frequency and time,reduced maze open arm percentage and open arm residence time percentage and increased serum ghrelin content(P<0.05).As compared with model group,in the peppermint oil treatment group were increased muscle tone,increased total distance,increased upright frequency and time,elevated maze open arm percentage and open arm residence time percentage,and decreased serum ghrelin levels(P<0.05).As compared with the model group,in the lavender essential oil treatment group were increased muscle tone,increased total distance and the total number of wear,reduced modify time,increased upright frequency and time,elevated maze open arm percentage and open arm residence time percentage,reduced serum ghrelin content,and the difference was statistically significant(P<0.05).Conclusions Lavender essential oil aromatherapy may improve the anxiety and activity of sleep-deprived rats by regulating the content of ghrelin in the body.

关 键 词：睡眠剥夺 薰衣草精油 抓握力 旷场 高架十字迷宫 胃饥饿素 瘦素 

分 类 号：R2-03[医药卫生—中医学]