血浆长链非编码RNA RNF5P1作为缺血性脑卒中生物标记物的研究  

Study on plasma lncRNA RNF5P1 as a biomarker for ischemic stroke

作　　者：温莹[1] 汤月芳 黄素丽[3] 张艳炜[4] 周丽[5] 李頔 柯跃斌[1] 程锦泉[1] 陈青山[2] WEN Ying;TANG Yue-fang;HUANG Su-li;ZHANG Yan-wei;ZHOU Li;LI Di;KE Yue-bin;CHENG Jin-quan;CHEN Qing-shan(Department of Molecular Epidemiology,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China;Department of Public Health and Preventive Medicine,Jinan University School of Medicine,Guangzhou 510632,China;Department of Environment and Health,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China;Department of Immunization Planning and Management,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China;Department of School Health,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China)

机构地区：[1]深圳市疾病预防控制中心分子流行病研究室,深圳518055 [2]暨南大学医学院公共卫生与预防医学系,广州510632 [3]深圳市疾病预防控制中心环境与健康所,深圳518055 [4]深圳市疾病预防控制中心免疫规划所,深圳518055 [5]深圳市疾病预防控制中心学校卫生所,深圳518055

出　　处：《中华疾病控制杂志》2020年第6期701-705,共5页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金面上项目(81573242);国家自然科学基金青年基金项目(81703312);深圳市科技计划项目(JCYJ20170306160036900)。

摘　　要：目的探讨血浆长链非编码RNA(lncRNA)RNF5P1表达水平与缺血性脑卒中(ischemic stroke,IS)发病风险的关联,评估血浆RNF5P1作为IS生物标记物的可行性。方法采用1∶1匹配的病例对照研究,在深圳地区选取302对性别年龄匹配的IS新发病例和正常体检对照,采用微滴式数字PCR检测血浆lncRNA RNF5P1的表达水平,采用条件Logistic回归分析模型分析RNF5P1表达水平与IS发病风险之间的关联,采用受试者工作特征曲线及重新分类的方法评估RNF5P1是否可作为IS的分子标记物。结果 IS病例组血浆lncRNA RNF5P1的表达水平高于对照组,差异有统计学意义(P<0.001)。高表达水平的RNF5P1与IS发病风险增加有关,校正其他危险因素后,OR值为2.52(95%CI:1.12~5.64)。与传统危险因素模型相比,加入lncRNA RNF5P1后,模型的曲线下面积增加(P<0.001),净重分类改善度为0.42(95%CI:0.26~0.57),综合区分改善度为0.24(95%CI:0.14~0.34)。结论血浆lncRNA RNF5P1表达水平升高与IS发病风险增加有关,lncRNA RNF5P1有望作为IS的生物标记物。Objective To explore the association between the expression level of plasma long non-coding RNA(lncRNA) RNF5 P1 and the risk of ischemic stroke(IS), and to examine the potential of RNF5 P1 as a biomarker for IS. Methods A 1∶1 matched case-control study with 302 pairs of newly diagnosed IS patients and controls was conducted in Shenzhen. LncRNA expression levels were measured using droplet digital polymerase chain reaction. Conditional logistic regression model was used to assess the association between plasma RNF5 P1 expression level and IS risk. The receiver operating characteristic(ROC) and reclassification analysis were conducted to assess the feasibility of using plasma RNF5 P1 as a novel biomarker for the detection of IS. Results The expression levels of lncRNA RNF5 P1 were signific-antly higher in the patients with IS than the controls(P<0.001). The higher expression level of RNF5 P1 was associated with increased risk of IS, and the adjusted odds ratio was 2.52(95% CI: 1.12-5.64). Addition of RNF5 P1 to the well-recognized risk factors led to significant improvement in the area under the ROC curve(P<0.001), with a net reclassification improvement of 0.42(95% CI:0.26-0.57) and an integrated discrimination improvement of 0.24(95% CI: 0.14-0.34). Conclusions The level of plasma RNF5 P1 is positively associated with the risk of IS. Our results suggest the potential for plasma lncRNA RNF5 P1 to be used as a novel molecular biomarker for IS.

关 键 词：缺血性脑卒中 长链非编码RNA 血浆 生物标记物 

分 类 号：R181[医药卫生—流行病学]