潞党参对环磷酰胺诱导蚕豆根尖的抗突变作用  

Anti-mutant Effect of Codonopsis pilosula on Vicia faba Root Tip Induced by Cyclophosphamide

作　　者：秦永燕[1] 赵青松 刘晨 刘建军 刘瑞祥[1] QIN Yongyan;ZHAO Qingsong;LIU Chen;LIU Jianjun;LIU Ruixiang(Department of Biological Science and Technology,Changzhi College,Changzhi 046011,China)

机构地区：[1]长治学院生物科学与技术系,山西长治046011

出　　处：《河南农业科学》2020年第7期53-57,共5页Journal of Henan Agricultural Sciences

基　　金：山西省高等学校科技创新项目(2019L0902);长治学院校级科研课题(zz201816);国家自然科学基金项目(31701125)。

摘　　要：为明确潞党参的抗突变作用,采用不同质量浓度(0.01、0.05、0.10、0.15、0.20 g/mL)潞党参水提液对30 mg/L环磷酰胺诱导的蚕豆根尖进行恢复培养,统计其微核数,并测定还原糖含量、根系活力、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果表明,与阳性对照(30 mg/L环磷酰胺处理后蒸馏水恢复培养)相比,不同质量浓度潞党参均可降低30 mg/L环磷酰胺处理下蚕豆根尖的微核率,增加根尖还原糖含量,增强根系活力,提高SOD活性并降低MDA含量。当潞党参质量浓度为0.05 g/mL时,蚕豆根尖的微核抑制率为35.41%,还原糖含量最高,为2.81%,根系活力最高,为120.73μg/(g·h),SOD活性最高,为88.44 U/g,MDA含量最低,为13.75 nmol/g。因此,潞党参对30 mg/L环磷酰胺诱导蚕豆根尖细胞损伤有一定的抗突变作用,且以质量浓度为0.05 g/mL时效果最好。To explore the anti-mutant effect of Codonopsis pilosula,the root tip of Vicia faba induced by 30 mg/L cyclophosphamide was cultured with different concentrations(0.01,0.05,0.10,0.15,0.20 g/mL)of water extract of Codonopsis pilosula,and the micronucleus was recorded,the content of reducing sugar,root activity,SOD activity,and MDA content were determined.The results showed that:Compared with the positive control(recovery culture by distilled water after 30 mg/L cyclophosphamide under treatment),different concentrations of Codonopsis pilosula decreased the micronucleus rate of Vicia faba root tip,increased the reducing sugar content of root tip,enhanced the root activity,increased the activity of SOD and decreased the MDA content under 30 mg/L cyclophosphamide treatment,when the concentration of Codonopsis pilosula was 0.05 g/mL,the inhibition rate of micronucleus in Vicia faba root tip was 35.41%,and the content of reducing sugar was the highest(2.81%),root activity was the highest[120.73μg/(g·h)],SOD activity was the highest(88.44 U/g),and MDA content was the lowest(13.75 nmol/g).Therefore,Codonopsis pilosula had a certain anti-mutant effect on the damage of Vicia faba root tip cells induced by 30 mg/L cyclophosphamide,and the effect was the best when the concentration was 0.05 g/mL.

关 键 词：潞党参 蚕豆 环磷酰胺 微核 根系活力 还原糖 超氧化物歧化酶 丙二醛 

分 类 号：S643.6[农业科学—蔬菜学]