1株鹅细小病毒的分离鉴定及致病特性、VP基因特征分析  

Isolation,Identification and Pathogenicity and VP Gene Characterization of Goose Parvovirus from Chengde White Goose

作　　者：刘宝山 李珮瑶 许莉莉 高晶萍 张瑞华[2] 徐彤[2] LIU Baoshan;LI Peiyao;XU Lili;GAO Jingping;ZHANG Ruihua;XU Tong(Luanping Agricultural and Rural Bureau,Luanping 068250,China;Key Laboratory of Preventive Veterinary Medicine,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]滦平县农业农村局,河北滦平068250 [2]河北北方学院预防兽医学重点实验室,河北张家口075000

出　　处：《河南农业科学》2020年第7期126-132,共7页Journal of Henan Agricultural Sciences

基　　金：河北省第二期现代农业产业技术体系蛋肉鸡创新团队建设专项(HBCT2018150207)。

摘　　要：为了解鹅细小病毒(GPV)在河北省的流行情况及分子遗传特征,对临床发病承德白鹅疑似小鹅瘟病料进行病毒分离鉴定及致病性、VP基因特征研究。结果显示,从病料中分离到1株GPV;人工感染6日龄雏鹅,感染后96 h全部死亡,临床症状和剖检变化与发病鹅相似;将测序序列拼接后得到VP基因片段,长度为2475 bp,包含VP1、VP2、VP3等3个开放阅读框,根据遗传进化分析和同源性分析结果发现,分离到的GPV(HB株)处于Ⅰb亚洲群;HB株与GPV各毒株VP基因相似性在96.6%~99.7%,其中与SHFX1201、Y株的氨基酸相似性最高,达99.7%。以上结果表明,HB株与国内分离GPV毒株之间差异较小,在分子生物学上证明了GPV只有1个血清型的观点。In order to understand the prevalence and molecular genetic characteristics of goose parvovirus(GPV)in Hebei Province,in this experiment,virus isolation,pathogenicity and VP gene characteristics were studied using clinically ill Chengde white goose.The results showed that a GPV strain was isolated from the disease material.Artificially infected 6-day-old goslings died at 96 h after infection,and clinical symptoms and necropsy changes were similar to those of the diseased goose.The sequence of the VP gene was spliced,and sequence with length of 2475 bp was obtained,including VP1,VP2,VP3 three open reading frames.According to genetic evolution analysis and homology analysis,it was found that the isolated GPV HB strain belonged to theⅠb Asian group in the genetic evolution tree.The similarity of VP gene between HB strain and other GPV strains was 96.6%—99.7%,and the amino acid similarity with SHFX1201 and Y strains was the highest(99.7%).The above results indicate that,the difference between the HB strain and the domestically isolated GPV strains is small,and the viewpoint that the GPV has only one serotype is confirmed in molecular biology.

关 键 词：承德白鹅 鹅细小病毒 致病特性 VP基因 分子特征 

分 类 号：S855.3[农业科学—临床兽医学]