量子点流式微球技术用于DNA的检测研究  

Quantum dots cytometric bead assay for DNA detection

作　　者：蔡冰洁 熊晓庆 Bing-jie Cai;Xiao-qing Xiong(Department of Clinical Laboratory,People's Hospital of Wuhan University,Wuhan,Hubei 430060,China;School of Tropical and Laboratory Medicine,Hainan Medical University,Haikou,Hainan 571199,China)

机构地区：[1]武汉大学人民医院检验科,湖北武汉430060 [2]海南医学院热带医学与检验医学院,海南海口571199

出　　处：《中国现代医学杂志》2020年第13期1-5,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81802111)。

摘　　要：目的构建一种基于新型纳米材料量子点的流式微球技术,用于高通量的DNA检测,实现DNA的快速、低成本检测。方法将能与靶DNA一端杂交的探针DNA(P1)标记在微球表面,与DNA配对杂交后,加入能与靶DNA另一端杂交的量子点标记的探针DNA(P2),组装成一种流式微球-探针P1、靶DNA、量子点-探针P2的夹心复合结构,通过测定杂交前后平均荧光强度的变化检测DNA的浓度。结果该新型检测方法可以区分出完全互补、单碱基错配及完全非互补的DNA(P<0.05),且随着DNA浓度的升高,检测到的平均荧光强度逐渐增强(P<0.05),对DNA的最低检出限可达0.2 nmol/L。结论新型量子点流式微球技术检测DNA具有高敏感性和高特异性、分析速度快、操作简单等优点,是一种非常重要的具有潜力的新型临床检测方法。Objective To detect DNA with high through,efficiency and low-cost,a novel nanomaterial based method established by combining quantum dots with cytometric bead assay.Methods The DNA probes(P1)which can hybridize with one end of target DNA are immobilized onto the surface of microsphere.After hybridizing with target DNA,quantum dots decorated probe DNA(P2)were introduced to hybridize with the other end of target DNA.Then a complex structure of microsphere-P1,target DNA and quantum dots-P2 was fabricated,the concentration of target DNA was detected by measuring changes in mean fluorescence intensity before and after hybridization.Results Complementary,one-base mismatched and non-complementary DNA can be distinguished by the novel method(P<0.05).With the increasing concentration of target DNA,the mean fluorescence intensity increased synchronously(P<0.05).The detection limit of target DNA can reach 0.2 nmol/L.Conclusions The DNA was detected by the novel method of quantum dots cytometric bead assay.It has the advantages of high sensitivity and specificity,rapid analysis,easy operation and so on.It is of great importance in point-of-care testing and is a promising novel method in clinical application.

关 键 词：量子点流式微球技术 DNA 快速检测 

分 类 号：R440[医药卫生—诊断学]