槲皮素对支气管哮喘小鼠气道炎症的作用及机制研究  

Inhibitory effect of quercetin on airway inflammation in mice with bronchial asthma

作　　者：朱翔 肖云斌[1] 易晓莲 Xiang Zhu;Yun-bin Xiao;Xiao-lian Yi(Department of Pediatrics,The Affiliated Hospital of Hangzhou Normal University,Hangzhou,Zhejiang 310015,China)

机构地区：[1]杭州师范大学附属医院儿科,浙江杭州310015

出　　处：《中国现代医学杂志》2020年第13期19-22,共4页China Journal of Modern Medicine

摘　　要：目的研究槲皮素是否对支气管哮喘小鼠模型气道炎症具有抑制作用及可能的分子机制。方法通过腹腔注射卵蛋白(OVA)致敏并行雾化激发复制小鼠支气管哮喘模型。将36只BALB/c雌性小鼠随机分为6组,分别为对照组(CON组)、哮喘模型组(OVA组)、槲皮素低剂量组(LOW组)、槲皮素中剂量组(MID组)、槲皮素高剂量组(HIGH组)剂量组、地塞米松阳性对照组(POS组)。对小鼠哮喘症状的程度进行评分;通过ELISA法检测小鼠支气管肺泡灌洗液(BALF)的上清液中白细胞介素-4(IL-4)和IL-5的水平;qRT-PCR检测肺组织miR-155的水平。结果与CON组比较,OVA组小鼠的哮喘症状评分较高,IL-4、IL-5和miR-155表达水平上调(均P<0.05)。与OVA组小鼠比较,随着槲皮素药物浓度的升高,槲皮素不同浓度组小鼠的哮喘症状评分下降,IL-4、IL-5和miR-155表达水平降低(均P<0.05)。结论槲皮素能改善支气管哮喘小鼠气道炎症,其机制可能与下调IL-4、IL-5和miR-155表达有关。Objective To investigate whether quercetin can inhibit airway inflammation in asthmatic mice and its specific molecular mechanism.Methods A mouse model of bronchial asthma was established by intraperitoneal injection of ovalbumin(OVA).Thirty-six female BALB/c mice were randomly divided into 6 groups:control group(CON group),asthma model group(OVA group),low dose quercetin(LOW group),middle dose quercetin(MID group),high dose quercetin(HIGH group)and dexamethasone positive control group(POS group).The asthmatic symptoms were scored in mice,the levels of inflammatory mediators IL-4 and IL-5 in the supernatant of bronchoalveolar lavage fluid(BALF)were detected by enzyme-linked immunosorbent assay(ELISA),and the levels of microRNA-155 in lung tissues were detected by qRT-PCR.Results Compared with CON group,OVA group had higher asthma symptom score and higher expression of IL-4,IL-5 and microRNA-155(all P<0.05).Compared with OVA group,the asthma symptom score decreased,and IL-4,IL-5 and microRNA-155 decreased with the increase of quercetin concentration(all P<0.05).Conclusion Quercetin inhibits the inflammatory mediators IL-4 and IL-5,ameliorate airway inflammation in mice with bronchial asthma,which may be related to the down-regulation of microRNA155.

关 键 词：支气管哮喘 槲皮素 气道炎症 炎症介质 

分 类 号：R562.25[医药卫生—呼吸系统]