BD Phoenix 100全自动微生物分析系统性能验证评价  

Performance verification and evaluation of BD Phoenix 100 automated microbiology system

作　　者：翁智 吴茳铃 余雪梅 李代昆 WENG Zhi;WU Jiangling;YU Xuemei;LI Daikun(Key Laboratory of Clinical Laboratory Diagnostics,Ministry of Education,School of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China;Department of Laboratory Medicine,University-Town Hospital of Chongqing Medical University,Chongqing 401331,China)

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016 [2]重庆医科大学附属大学城医院检验科,重庆401331

出　　处：《检验医学与临床》2020年第S01期1-5,共5页Laboratory Medicine and Clinic

基　　金：重庆市卫生和计划生育委员会医学科研项目(2017MSXM087)。

摘　　要：目的进行BD Phoenix 100全自动微生物分析系统的鉴定和药敏试验性能评价。方法对15株菌株进行30次鉴定(每株检测2次),对金黄色葡萄球菌(ATCC29213)、大肠埃希菌(ATCC25922)、铜绿假单胞菌(ATCC27853)进行90次药敏试验(每日3次,连续5 d,再重复1次)。分析BD Phoenix 100全自动微生物分析系统的鉴定准确度和精密度、药敏试验准确度和精密度、检测时间。结果BD Phoenix 100全自动微生物分析系统的鉴定准确度为97.73%、精密度为100.00%,药敏试验准确度为99.89%、精密度为95.96%,检测时间为13.90 h。结论BD Phoenix 100全自动微生物分析系统的鉴定和药敏试验的准确度和精密度都很高,检测时间比较短,适用于临床微生物的准确、快速鉴定,能为临床合理使用抗菌药物提供可靠的参考数据。Objective To evaluate the performance of BD Phoenix 100 automated microbiology system.Methods Identification of 15 bacteria were done for 30 times(2 times for each strain),antimicrobial susceptibility test of Staphylococcus aureus(ATCC 29213),Escherichia coli(ATCC 25922)and Pseudomonas aeruginosa(ATCC 27853)were done for 90 times(3 times a day,5 consecutive days,repeat once again).The identification accuracy and precision,drug sensitivity test accuracy and precision,and detection time of BD Phoenix 100 automatic microbial analysis system were analyzed.Results The identification accuracy and precision of D Phoenix 100 automatic microbiological analysis system were 97.73%and 100.00%,and the sensitivity test accuracy and precision were 99.89%and 95.96%,respectively,detection time was 13.90 hours.Conclusion BD Phoenix 100 automatic microbiological analysis system has high accuracy and precision in identification and drug sensitivity test and detection time was short,the analyzer is available for clinical microbial ID and AST and can provide significant data for antimicrobials usage.

关 键 词：BD Phoenix 100 鉴定 抗菌药物药敏试验 性能评价 

分 类 号：R44[医药卫生—诊断学]