lncRNA RP11-86H7.1在川崎病诊断中的临床意义  

Clinical significance of lncRNA RP11-86H7.1 in the diagnosis of Kawasaki disease

作　　者：潘璐璐[1,2] 卢孔场 褚茂平 曾静静[1] 荣星[1] PAN Lulu;LU Kongchang;CHU Maoping;ZENG Jingjing;RONG Xing(Children’s Heart Center,the Second Affiliated Hospital&Yuying Children’s Hospital,Institute of Cardiovascular Development&Translational Medicine,Wenzhou Medical University,Wenzhou 325027,China;Department of Child Health Management,Wenzhou Women and Child Health Station,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第二医院育英儿童医院儿童心脏中心温州医科大学心脏发育与转化医学研究所,浙江温州325027 [2]温州市妇幼保健所儿童健康管理科,浙江温州325000

出　　处：《温州医科大学学报》2020年第8期628-631,636,共5页Journal of Wenzhou Medical University

基　　金：浙江省医药卫生科技计划项目(2016KYB197);温州市科技计划项目(Y20150015)。

摘　　要：目的:探讨lncRNA RP11-86H7.1在川崎病(KD)患者血清中的表达及其与临床病理特征及预后的关系。方法:筛选KD特异相关的循环lncRNA,分KD治疗前患儿组、KD治疗后患儿组、普通发热患儿组及健康儿童组,采用qPCR检测各组血清lncRNA RP11-86H7.1相对表达。分析血清lncRNA RP11-86H7.1相对表达与KD临床病理特征间关系;绘制ROC曲线,分析血清lncRNA RP11-86H7.1表达水平对KD的诊断效能。结果:KD急性患儿组血清lncRNA RP11-86H7.1相对表达量高于各对照组(P<0.05);年龄和性别比例与低表达组比较差异无统计学意义(P>0.05);qPCR发现lncRNA RP11-86H7.1在KD急性期患儿血清中表达水平明显高于KD恢复期、健康儿童及发热儿童组,差异均有统计学意义(P<0.05)。结论:血清lncRNA RP11-86H7.1在KD患者中表达上调,其可作为KD早期诊断和评估预后的潜在的生物标志物。Objective:To investigate the expression of lncRNA RP11-86H7.1 in serum of Kawasaki disease(KD)patients and its correlation with clinicopathological characteristics and prognosis.Methods:The circulating lncRNA specifically related to KD were screened and divided into four groups:children before KD treatment,children after KD treatment,children with common fever and healthy children group.The results of lncRNA microarray showed that excluding the influence of fever,the expression of lncRNA in plasma of kawasaki patients was higher than that in normal children and during recovery period.The relative expression of serum lncRNA RP11-86H7.1 in the normal children group and the group before KD treatment was detected by real-time fluorescence quantitative PCR.The correlation between the expression of serum lncRNA RP11-86H7.1 and the clinicopathological characteristics of kawasaki disease was analyzed;ROC curve was drawn to analyze the diagnostic effect of serum lncRNA RP11-86H7.1 expression level on kawasaki disease.Results:The relative expression of serum lncRNA RP11-86 H7.1 in the children’s group before KD treatment was higher than that in the control group(P<0.05).There was no significant difference in age and sex ratio between the low-expression group and the low-expression group(P>0.05).Real-time PCR showed that the expression level of lncrnarp11-86H7.1 in serum of KD patients in acute stage was significantly higher than that in KD recovery stage,normal control group and fever control group.The differences were statistically significant(P<0.05).Conclusion:Serum lncRNA RP11-86H7.1 was up-regulated in patients with KD,and it can be used as a potential biomarker for early diagnosis and prognosis evaluation of KD.

关 键 词：川崎病 lncRNA RP11-86H7.1 诊断 

分 类 号：R725.4[医药卫生—儿科]