大珠母贝胰岛素相关肽受体基因的克隆与表达  

Gene Cloning and Expression Pattern Analysis of Insulin-related Peptide Receptor from Pinctada maxima

作　　者：赵子涵 王姿曼 邓岳文[1,2] 杨创业 李俊辉[1] ZHAO Zi-han;WANG Zi-man;DENG Yue-wen;YANG Chuang-ye;LI Jun-hui(Fisheries College of Guangdong Ocean University,Zhanjiang 524088,China;Pearl Technology and Innovation Center of Guangdong Province,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524025 [2]广东省珍珠科技创新中心,广东湛江524025

出　　处：《广东海洋大学学报》2020年第5期34-42,共9页Journal of Guangdong Ocean University

基　　金：农业产业技术体系专项(CARS-49);广东省教育厅珍珠研究创新团队(2017KCXTD016);广东省农业农村厅现代农业产业技术创新团队专项(2019KJ146)。

摘　　要：【目的】克隆大珠母贝(Pinctada maxima)胰岛素相关肽受体PmIRR基因,并分析其在不同组织中的表达。【方法】利用c DNA快速末端克隆技术(RACE)获得大珠母贝PmIRR基因cDNA全长序列。荧光定量PCR技术分析PmIRR在闭壳肌、鳃、外套膜边缘区、套膜区、中央区、足和肝胰腺等组织的表达模式。【结果与结论】PmIRR基因全长6 009 bp,5′非编码区(UTR)615 bp、3′UTR 764 bp、开放阅读框(ORF)长4 629 bp、编码1 542个氨基酸。序列分析表明,PmIRR含有保守结构域Fu、3个FNⅢ结构域和Tyrkc结构域,并含有信号肽和2个跨膜结构域。PmIRR在大珠母贝各个组织存在差异表达,在鳃、肝胰腺和外套膜边缘区中显著高表达。【Objective】The insulin-related peptide receptor(PmIRR)gene of Pinctada maxima was cloned and its expression patterns in various tissues were analyzed.【Method】The full-length cDNA sequence of PmIRR was cloned by rapid amplification of cDNA ends(RACE)and the tissue expression pattern of this gene was determined by RT-PCR.【Result and conclusion】The full-length of PmIRR was 6009 bp.It consist of a 5′UTR(615 bp),a 3′UTR(764 bp)and an open reading frame(4627 bp)which encoded for 1542 amino acids.d Amino acids analysis of PmIRR showed that it contained a conservative domain Fu,three FNIII domains,a tyrkc domains,a signal peptide and two transmembrane domains.PmIRR was differentially expressed in different tissues of Pinctada maxima,and the expression was significantly higher in gills,hepatopancreas and mantle edge.

关 键 词：大珠母贝 胰岛素肽相关受体 基因克隆 表达模式 

分 类 号：Q78[生物学—分子生物学] Q959.215.4