3种荒漠珍稀植物根际促生菌的筛选、鉴定及对高粱幼苗生长的影响  

Screening and Identification of Growth-promoting Bacteria from Three Rare Plants Rhizosphere Soil in Desert and Its Effect on the Growth of Sorghum Seedlings

作　　者：郭雨晴 赵世超 徐道龙 郭洋楠[1] 贺安民[1] 常建鸿 包玉英 GUO Yu-qing;ZHAO Shi-chao;XU Dao-long;GUO Yang-nan;HE An-min;CHANG Jian-hong;BAO Yu-ying(The Technology Research Institute of Shendong Coal Company in China Shenhua Group,Ordos, Inner Mongolia 017200, China;Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, State Key Laboratory of Reproductive Regulatory and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010010, China)

机构地区：[1]中国神华能源股份有限公司神东煤炭分公司技术研究院,内蒙古鄂尔多斯017200 [2]内蒙古大学生命科学学院牧草与特色作物生物技术教育部重点实验室,省部共建草原家畜生殖调控与繁殖国家重点实验室,内蒙古呼和浩特010071

出　　处：《草地学报》2020年第4期1121-1128,共8页Acta Agrestia Sinica

基　　金：神东煤炭分公司科技创新项目(201691548939);内蒙古自然科学基金项目(2015ZD04);内蒙古自治区科技厅重大项目(ZDZX2018065);国家自然科学基金(30960309)资助。

摘　　要：为获得优良植物根际促生菌,本研究从荒漠珍稀植物根际土中分离筛选促生菌,结合16S rDNA基因序列、生理生化特征对其进行鉴定,并通过发芽和盆栽试验分析其促生效果。结果显示:土壤全氮、有效钾、有机质含量较少,呈弱碱性;分离得到5株可固氮、溶磷、产蛋白酶的菌株BN5,HN7,BP4,HD3及SD5,其分别隶属于鞘氨醇单胞菌属(Sphingomonas sp.)、根癌农杆菌属(Agrobacterium tumefaciens)、节杆菌属(Arthrobacter sp.)、磷酸镰孢菌属(Fictibacillus phosphorivorans)及单纯芽孢杆菌属(Bacillus simplex);5株菌对高粱(Sorghum bicolor)幼苗均表现出促生作用,效果最明显的为菌株BP4,其处理的高粱幼苗发芽势、发芽率、芽长、根长、株高及鲜重较对照组分别增加了55.30%,23.16%,54.52%,40.24%,22.59%,26.67%。本研究结果可为今后荒漠优良菌种资源库的建设提供理论依据。In order to obtain excellent plant growth promoting rhizobacteria,growth-promoting strains were isolated and screened from rare plants rhizosphere soil in desert.Strains were identified by 16S rDNA gene sequence and physiological and biochemical characteristics,and analyzed their growth-promoting effect by germination and pot experiments.The results showed that the concentrations of total nitrogen,available potassium and organic matter were extremely deficient in rhizosphere soil,and the soil were weakly alkaline.We isolated 5 strains(BN5,HN7,BP4,HD3 and SD5)which had the ability to fix nitrogen,solubilize phosphorus and produce proteinase.They were belong to Sphingomonas sp.,Agrobacterium tumefaciens,Arthrobacter sp.,Fictibacillus phosphorivorans and Bacillus simplex,respectively.They all showed growth-promoting ability on Sorghum seedlings,and BP4 was the best.The germination potential,germination rate,bud length,root length,plant height and fresh weight of Sorghum seedlings treated by BP4 increased by 55.30%,23.16%,54.52%,40.24%,22.59%,and 26.67%,respectively,when compared with the control group.These results provide a theoretical basis for research on the construction of an excellent desert microbiological resource bank in the future.

关 键 词：荒漠珍稀植物 根际促生菌 固氮 溶磷 产蛋白酶 

分 类 号：Q939.9[生物学—微生物学] Q948.1