“双固一通”灸法对糖尿病及糖尿病周围神经病变大鼠胰岛素抵抗的影响  

Effects of“Shuanggu Yitong”Moxibustion Therapy on IR in Rats with DM and DPN

作　　者：曾林 向婷[2] 王天沛 胡霞[1] 唐宏图[1,3] ZENG Lin;XIANG Ting;WANG Tianpei;HU Xia;TANG Hongtu(Hubei University of Chinese Medicine, Wuhan 430065, China;Wuhan General Hospital of Guangzhou Military Area Command of Chinese PLA, Wuhan 430070, China;Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430061, China)

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]解放军广州军区武汉总医院,湖北武汉430070 [3]针灸治未病湖北省协同创新中心,湖北武汉430061

出　　处：《针灸临床杂志》2020年第5期81-85,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：湖北省教育厅科学研究计划重点项目,编号:D20142006。

摘　　要：目的:观察“双固一通”灸法对糖尿病及糖尿病周围神经病变大鼠血脂代谢、炎性因子分泌及肝脏组织中胰岛素受体(InsR)基因表达的影响。方法:大鼠分为正常组(A组)、糖尿病模型组(B组)、糖尿病模型艾灸治疗组(C组)、糖尿病周围神经病变模型组(D组)、糖尿病周围神经病变模型艾灸治疗组(E组),于4周、8周治疗结束后空腹眼底静脉采血测各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C),于治疗4周、8周后,用ELISA法测定血清肿瘤坏死因子TNF-α、白介素IL-6的含量,以及运用实时定量聚合酶链式反应方法检测肝脏胰岛素受体InsR的含量。结果:C组与B组相比,TC、TG、LDL-C水平降低(P<0.05),HDL-C水平升高(P<0.05);E组与D组相比,TC、TG、LDL-C水平降低(P<0.05),HDL-C水平升高(P<0.05)。B组和D组血清肿瘤坏死因子TNF-α与白介素IL-6的含量显著高于A组(P<0.05);与B组比较,C组大鼠TNF-α与IL-6的含量均降低(P<0.05);与D组比较,E组大鼠TNF-α、IL-6的含量均降低(P<0.05)。B组和D组肝脏胰岛素受体InsR表达明显低于A组(P<0.05);C组与B组相比,InsR表达明显增加(P<0.05);E组与D组相比,InsR表达明显增加(P<0.05)。结论:“双固一通”艾灸法治疗后,改善了糖尿病及周围神经病变大鼠血脂紊乱以及炎性因子分泌的情况,也使得糖尿病与周围神经病变大鼠的胰岛素受体数量增加、功能增强,改变了胰岛素受体数量和功能缺陷所导致的胰岛素作用减弱的状态,进而改善胰岛素抵抗情况,这可能也是“双固一通”艾灸法防治糖尿病及糖尿病周围神经病变的作用机制之一。Objective:To observe the effect of"Shuanggu Yitong"moxibustion therapy on lipid metabolism,inflammatory factor secretion and gene expression of insulin receptor(InsR)in rats with diabetes mellitus(DM)and diabetic peripheral neuropathy(DPN).Methods:Rats were randomly divided into the normal group,the DM model group,the DM treatment group,the DPN model group and the DPN treatment group.The serum levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)were tested after four weeks and after eight weeks of treatment.The serum contents of TNF-a and IL-6 were detected by ELISA method.The content of insulin receptor InsR in liver was detected by the method of real-time quantitative polymerase chain reaction.Results:The levels of TC,TG and LDL-C were significantly decreased and the level of HDL-C was significantly increased in the DM treatment group compared to those in the DM model group(P<0.05).Also,the levels of TC,TG and LDL-C were significantly decreased and the level of HDL-C was significantly increased in the DPN treatment group compared to those in the DPN model group(P<0.05).The serum levels of TNF-a and IL-6 were obviously higher in the DM model group and in the DPN model group compared to those in the normal group(P<0.05),which were significantly lowered after corresponding intervention in the DM treatment group and in the DPN treatment group(P<0.05).The expression of insulin receptor InsR in liver was significantly lower in the DM model group and in the DPN model group compared to that in the normal group(P<0.05),which were significantly increased after corresponding intervention in the DM treatment group and in the DPN treatment group(P<0.05).Conclusion:“Shuanggu Yitong”moxibustion therapy can improve the blood lipid disorders and inflammatory factor secretion in the rats with DM and DPN.It can also increase the number of insulin receptors and enhance their functions,it improves the weakened effect of insulin caused by the redu

关 键 词：双固一通 灸法 糖尿病 周围神经病变 基础研究 

分 类 号：R246.1[医药卫生—针灸推拿学]