脊尾白虾甘露糖结合凝集素(MBL)基因在抗镉胁迫中的生物学功能分析  

Biological Functional Analysis of MBL Gene in Resistance to Cadmium Stress in Exopalaemon carinicauda

作　　者：徐莞媛 马杭柯 孙金秋 段健诚 邓高威 高焕[1,2,3,4] 阎斌伦 XU Wanyuan;MA Hangke;SUN Jinqiu;DUAN Jiancheng;DENG Gaowei;GAO Huan;YAN Binlun(Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005;Co-Innovation Center of Jiangsu Marine Bio-Industry Technology,Huaihai Institute of Technology,Lianyungang 222005;Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014;Marine Resource Development Institute of Jiangsu(Lianyungang),Lianyungang 222005)

机构地区：[1]江苏省海洋生物资源与生态环境重点实验室,江苏省海洋生物技术重点实验室淮,海工学院,连云港222005 [2]江苏省海洋生物产业技术协同创新中心,连云港222005 [3]江苏省农业种质资源保护与利用平台,南京210014 [4]江苏省海洋资源开发研究院(连云港),连云港222005

出　　处：《渔业科学进展》2020年第4期174-180,共7页Progress in Fishery Sciences

基　　金：江苏省高等学校自然科学研究重大项目(17KJA240001);2017年淮海工学院研究生科研创新计划项目(XKYCXX2017-11);江苏省优势学科建设工程项目共同资助。

摘　　要：为研究Cd^2+胁迫下脊尾白虾(Exopalaemoncarinicauda)甘露糖集合凝集素(MBL)在肝胰腺中表达量的变化特征,本研究利用重金属镉(Cd^2+)对脊尾白虾进行96 h急性毒性实验。实验共设置5组Cd^2+浓度胁迫(0、0.01、0.0175、0.021和0.0278mmol/L),分别在Cd^2+胁迫后0、3、6、12、24、48、72和96h共8个时间点取样。实时荧光定量结果显示,高浓度Cd^2+(0.0175、0.021和0.0278 mmol/L)胁迫下,脊尾白虾MBL基因的表达量先呈上升趋势,在72 h达到峰值后随后下降,但各时间点表达量均与对照组存在显著性差异(P<0.01);Cd^2+浓度为0.01 mmol/L时,脊尾白虾MBL基因的表达量整体呈下降趋势。进一步采用RNA技术干扰该基因表达后,发现Cd^2+胁迫后的脊尾白虾个体死亡率显著增加。本研究表明,脊尾白虾MBL在应答Cd^2+胁迫过程中可对机体起到一定的保护作用。In order to study the expression profile of mannose-binding lectin(MBL)in response to the stress of heavy metal cadmium(Cd^2+)in the ridgetail white prawn,Exopalaemon carinicauda,changes of MBL expression level during 96 h of stress were analyzed in the tissue of hepatopancreas.Five Cd^2+ stress concentrations(0,0.01,0.0175,0.021 and 0.0278 mmol/L)were set up in the experiment,and the samples were taken at 8 time points,i.e.0,3,6,12,24,48,72 and 96 hours after Cd^2+ stress.The real-time fluorescence quantitative results showed that under the stress of high concentration of Cd^2+(0.0175,0.021 and 0.0278 mmol/L),the expression level of MBL gene in the ridgetail white prawn increased first,then decreased after reaching the peak at 72 h.There are significant differences between experimental group and control group at all the stress time points.When the cadmium ion was 0.01 mmol/L,a lower concentration,the expression level of MBL gene in the ridgetail white prawn tends to decrease from the beginning.Further interfering down the expression level of MBL using the RNA technology,it was discovered that the mortality rate of Cd^2+ stressed individuals were higher than those of the control group.This study showed that MBL potentially took part in the protection of E.carinicada from Cd^2+ stress.

关 键 词：脊尾白虾 MBL 镉 RNA干扰技术 急性毒性实验 

分 类 号：S917.4[农业科学—水产科学]