陆地棉NF-YA基因家族的全基因组鉴定与功能分析  

Genome-Wide Identification and Functional Analysis of the NF-YA Gene Family in Gossypium hirsutum L.

作　　者：潘奥 王静静 孙福来 张景霞[2] 高阳[2] 杜召海 焦梦佳 张军[2] 王芙蓉[2] 刘志[1] Pan Ao;Wang Jingjing;Sun Fulai;Zhang Jingxia;Gao Yang;Du Zhaohai;Jiao Mengjia;Zhang Jun;Wang Furong;Liu Zhi(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China;Key Laboratory of Cotton Genetic Improvement and Cultivation Physiology in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Shandong Cotton Research Center,Jinan 250100,China;Binzhou Seed Administration Station,Binzhou,Shandong 256618,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]农业农村部黄淮海棉花遗传改良与栽培生理重点实验室/山东棉花研究中心,济南250100 [3]山东省滨州市种子管理站,山东滨州256618

出　　处：《棉花学报》2020年第4期316-328,共13页Cotton Science

基　　金：国家现代农业产业技术体系棉花体系(CARS-15-05);国家自然科学基金(31671742和31601345);泰山学者计划(No.ts201511070);山东省农科院创新工程(CXGC2016A01)。

摘　　要：【目的】通过对陆地棉NF-YA基因家族进行全基因组鉴定,分析其表达特性,鉴定其中与棉花开花相关的基因。【方法】利用生物信息学方法系统分析了其理化性质、基因结构、共线性、Ka/Ks、顺式作用元件和表达模式,实时荧光定量聚合酶链反应(Polymerase chain reaction,PCR)分析表达特征,采用病毒诱导基因沉默技术验证基因功能。【结果】陆地棉中鉴定到29个NF-YA基因家族成员,分为5个亚组,定位在18条染色体上;全基因组复制和片段复制是GhNF-YA基因家族扩张的主要动力;启动子区含有大量光反应的顺式作用元件。GhNF-YA基因家族基因在茎、叶中高表达;12个GhNF-YA基因在早熟品种鲁棉研19号和晚熟品种鲁棉研37号中均在第三至六叶时期表达量较高,并且大部分基因在品种间表达差异显著。沉默GhNF-YA18基因的鲁棉研37号植株比对照提前11 d现蕾,并且该基因在白化期和现蕾期的表达量均低于未沉默对照棉株。【结论】本研究对陆地棉NF-YA基因家族进行了鉴定和表达分析,为进一步研究棉花开花调控的分子机制奠定了基础。[Objective]The NF-YA gene family was identified in upland cotton(Gossypium hirsutum L.),and their expression characteristics were analyzed to discover the genes related to cotton flowering regulation.[Method]Using bioinformatic methods,the NF-YA genes were identified and their physical and chemical properties,gene structure,cis-element,collinearity,Ka/Ks were investigated.The expression pattern was performed using quantitative real-time polymerase chain reaction(qRT-PCR).The gene function was verified by virus-induced gene silencing(VIGS).[Result]A total of 29 GhNF-YA genes were identified from upland cotton genome,these genes were divided into five clades and distributed on 18 chromosomes.The expansion of the NF-YA genes in upland cotton was mainly driven by whole genome duplication and segmental duplication.A lot of light responding elements were identified in the promoter regions of GhNF-YA genes.According to the expression profiling,the GhNF-YA genes were highly expressed in stems and leaves.Meanwhile,analysis of qRT-PCR showed that 12 GhNF-YA genes had a relatively high expression in the leaf of both early-maturing cotton cultivar Lumianyan 19 and late-maturing cotton cultivar Lumianyan 37 from three-to six-leaf stage,and expression of the most genes was significantly different between the two cultivars.The function of GhNF-YA18 in Lumianyan 37 was verified by VIGS,and the results indicated that the bud emergence in silenced plants was 11 d earlier than that of unsilenced control plants.In addition,the expression of GhNF-YA18 was lower in silenced plants than unsilenced plants at albino and budding stages.[Conclusion]In this study,we identified and characterized the 29 GhNF-YA genes in upland cotton.The results will provide a foundation for future studies in molecular regulatory mechanisms of cotton early fiowering.

关 键 词：陆地棉 NF-YA基因家族 生物信息学 表达分析 病毒诱导基因沉默(Virus-induced gene silencing VIGS) 

分 类 号：S562[农业科学—作物学]