社区和医院获得性耐甲氧西林金黄色葡萄球菌耐药基因及耐消毒剂基因的检测  

Detection of drug resistance gene and disinfectant resistance gene of methicillin-resistant Staphylococcus aureus in community and hospital

作　　者：向蓉 贾潇岳 陈光辉 陈荣忠 XIANG Rong;JIA Xiao-yue;CHEN Guang-hui;CHEN Rong-zhong(Affiliated Xiaolan Hospital of Southern Medical University,Zhongshan Guangdong 528415;Army Medical University,China)

机构地区：[1]南方医科大学附属小榄医院,广东中山528415 [2]陆军军医大学

出　　处：《中国消毒学杂志》2020年第6期436-440,共5页Chinese Journal of Disinfection

基　　金：广东省医学科学技术研究基金项目(B2016117);中山市科技局计划项目(2015B1077)。

摘　　要：目的了解伤口分泌物分离的社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)与医院获得性耐甲氧西林金黄色葡萄球菌(HA-MRSA)的耐药特点,探讨其耐药基因及耐消毒剂基因的携带情况。方法收集某院2016年1月-2017年12月伤口分泌物分离的耐甲氧西林金黄色葡萄球菌(MRSA)76株,采用聚合酶链反应(PCR)方法检测其耐药基因和耐消毒剂基因。结果共分离到MRSA 76株,其中CA-MRSA 52株,HA-MRSA 24株。52株CA-MRSA对四环素、庆大霉素、红霉素的耐药率分别为36.54%、1.92%、86.54%,24株HA-MRSA对四环素、庆大霉素、红霉素的耐药率分别为50.00%、12.50%、75.00%,HA-MRSA和CA-MRSA中,耐药基因tetM、ermA及耐消毒剂基因(qacA/B)的携带率均较高。结论伤口分泌物分离的CA-MRSA与HA-MRSA菌株耐药严重,均可携带多个耐药基因和耐消毒剂基因,应引起重视。Objective To understand the drug resistance characteristics of community-acquired methicillin-resistant Staphylococcus aureus(CA-MRSA)and hospital-acquired methicillin-resistant Staphylococcus aureus(HA-MRSA)isolated from wound secretions in this region,and to explore the carrying of drug resistance genes and disinfectant resistance genes.Methods 76 strains of methicillin-resistant Staphylococcus aureus(MRSA)isolated from the wound secretion of a hospital from January 2016 to December 2017 were collected,and their drug resistance genes and disinfectant resistance gene were detected by polymerase chain reaction(PCR).Results A total of 76 strains of MRSA were isolated in this study,including 52 strains of CA-MRSA and 24 strains of HA-MRSA.The resistance rates of 52 strains of CA-MRSA to tetracycline,gentamicin and erythromycin were 36.54%,1.92%and 86.54%.The resistance rates of 24 strains of HA-MRSA to tetracycline,gentamicin and erythromycin were 50.00%,12.50%and 75.00%.The detection rates of tetM,ermA and carrying rate of the disinfectant resistance gene(qacA/B)of 76 strains of MRSA were high.Conclusion The CA-MRSA and HA-MRSA strains isolated from wound secretions have serious drug resistance carry multiple drug resistance genes and disinfectant resistance genes,which should be paid attention to by the health department.

关 键 词：耐甲氧西林金黄色葡萄球菌 耐药基因 耐消毒剂基因 

分 类 号：R440[医药卫生—诊断学]