质谱法分析水及三种生物基质中的敌草快  

Determination of Diquat in blank water and three blank biological matrix with UPLC-MS/MS

作　　者：赵森[1] 王炯[2] 于忠山[2] Zhao Sen;Wang Jiong;Yu Zhongshan(Zhejiang Police College,Hangzhou,310053;Institute of Forensic Science of China,Beijing,100038)

机构地区：[1]浙江警察学院,浙江杭州310053 [2]公安部物证鉴定中心,北京100038

出　　处：《中国法医学杂志》2020年第3期274-277,共4页Chinese Journal of Forensic Medicine

基　　金：浙江省毒品防控技术研究重点实验室开放课题《基于城市污水流行病学法进行毒情分析和溯源研究》(2019005)。

摘　　要：目的建立了超高效液相色谱——质谱法(UPLC-MS/MS)测定环境样品水基质以及包括人血液、尿液、肝脏的生物样品中敌草快的检测方法。方法空白水基质、空白血、空白尿、空白肝加入敌草快标准溶液,经混合型弱阳离子交换柱(WCX)进行纯化,提取后采用UPLC-MS/MS检测。质谱检测采用正离子扫描,多反应离子监测模式(MRM)。结果空白水、空白血、空白尿、空白肝加标回收率在70%~100%,检出限均为0.1ng/mL。结论该方法灵敏度高,分析速度快,操作简便。Objective To establish a UPLC-MS/MS method for the quantitative determination and confirmation of Diquat.Methods The blank water,blank whole blood,blank urine and blank liver were vortexed and centrifuged.The supernatant was cleaned-up using Oasis®WCX solid phase extraction cartridges.Extracts were analyzed by UPLC-MS/MS in positive ionization and multiple reaction monitoring(MRM)mode.Results The average recovery of Diquat from the blank water,blank whole blood,blank urine and blank liver were 70%~100%.The detection limit was 0.10ng/mL.Conclusion The method is proved to be selective,sensitive and rapid.

关 键 词：敌草快 超高效液相色谱——质谱法 环境样品 生物基质 

分 类 号：DF795.2[医药卫生—法医学]