高龄反复自然流产患者封闭抗体和淋巴细胞亚群分析  

Analysis of blocking antibody and lymphocyte subsets in elderly patients with recurrent spontaneous abortion

作　　者：高景波 郭慧敏[1] 朱镭[1] 张晋丽[1] 杨波 郝超群 徐凯 Gao Jingbo;Guo Huimin;Zhu Lei;Zhang Jinli;Yang Bo;Hao Chaoqun;Xu Kai(Clinical Laboratory Examination Center,Children′s Hospital of Shanxi,Taiyuan 030013,China)

机构地区：[1]山西省儿童医院临检中心,太原030013

出　　处：《中国综合临床》2020年第4期376-379,共4页Clinical Medicine of China

摘　　要：目的探讨高龄(年龄≥35岁)反复自然流产(recurrent or repetitive spontaneous abortion,RSA)患者与封闭抗体和淋巴细胞的关系。方法选择2014年10月至2019年9月在山西省儿童医院妇幼保健院生殖中心就诊且符合纳入条件的RSA患者,进行回顾性病例对照研究。按年龄不同分为高龄RSA组66例,正常孕龄RSA组334例,另收集179名正常体检妇女作为对照。采用流式细胞术检测封闭抗体和淋巴细胞亚群,统计分析检查结果。结果高龄RSA患者封闭抗体阴性率为77.27%(51/66),与正常孕龄RSA患者封闭抗体阴性率83.53%(279/334)比较差异无统计学意义(P=0.221),显著高于正常妇女封闭抗体阴性率23.46%(42/179)(P=0.001);封闭抗体封闭效率结果显示高龄RSA患者CD3封闭效率0.10(-0.50,0.60)显著低于正常妇女CD3封闭效率0.60(0.00,1.30),差异有统计学意义(P=0.001);高龄RSA患者CD8封闭效率0.00(-0.60,0.63)显著低于正常妇女CD8封闭效率0.30(0.00,0.70),差异有统计学意义(P=0.016)。淋巴细胞亚群结果显示高龄RSA患者CD8+T细胞比率(26.93±7.25)显著低于正常孕龄RSA患者(29.22±7.29),差异有统计学意义(P=0.020);高龄RSA患者CD4+/CD8+比值(1.64±0.99)显著高于正常孕龄RSA患者(1.37±0.50),差异有统计学意义(P=0.030)。高龄RSA患者自然杀伤细胞比率(16.13±7.10)显著高于正常妇女(14.04±2.35),差异有统计学意义(P=0.022),且高于正常孕龄RSA妇女(15.57±7.02)。结论高龄RSA患者与正常孕龄RSA患者淋巴细胞亚群存在差异;自然杀伤细胞比率升高可能是RSA发生的重要因素,尤其是高龄RSA患者。Objective To explore the relationship between blocking antibody and lymphocyte subsets in elderly patients(age≥35 years old)with recurrent spontaneous abortion(RSA).Methods A retrospective case-control study was conducted on the patients with recurrent spontaneous abortion who met the inclusion criteria from October 2014 to September 2019 in the Reproductive Center of Women Health Center of Shanxi.They were divided into two groups according to their ages:66 cases in RSA group and 334 cases in normal pregnant age group.The blocking antibody and lymphocyte subsets were detected by flow cytometry.Results The negative rate of blocking antibody in elderly recurrent spontaneous abortion patients was 77.27%(51/66),which was not significantly different from that in normal gestational recurrent spontaneous abortion patients(83.53%(279/334))(P=0.221),but significantly higher than that in normal women(23.46%(42/179))(P=0.001).The blocking antibody′s blocking efficiency results showed that the CD3′s blocking efficiency of elderly recurrent spontaneous abortion patients(0.10(-0.50,0.60))was significantly lower than that of normal women(0.60(0.00,1.30)),the difference was statistically(P=0.001).The CD8 blocking efficiency of elderly recurrent spontaneous abortion patients(0.00(-0.60,0.63))was significantly lower than that of normal women(0.30(0.00,0.70)),the difference was statistically(P=0.016).Lymphocyte subsets showed that the CD8+T cell ratio in elderly recurrent spontaneous abortion patients(26.93±7.25)was significantly lower than that in normal gestational recurrent spontaneous abortion patients(29.22±7.29),the difference was statistically significant(P=0.020).The CD4+/CD8+ratio in elderly recurrent spontaneous abortion patients(1.64±0.99)was significantly higher than that in normal gestational age recurrent spontaneous abortion patients(1.37±0.50),the difference was statistically significant(P=0.030).The ratio of natural killer cells in elderly recurrent spontaneous abortion patients(16.13±7.10)was significan

关 键 词：反复自然流产 高龄 封闭抗体 淋巴细胞亚群 

分 类 号：R714.21[医药卫生—妇产科学]