LED补光对香蕉组培苗增殖和生理生化指标的影响  

Effects of LED supplementation on proliferation,and physiological and biochemical indices of tissue-cultured banana plantlets

作　　者：项蕾蕾 李丹 孙雪丽[1] 田娜 刘范 付帅 陈裕坤[1] 林玉玲[1] 程春振[1] 王天池[1] 赖钟雄[1] XIANG Leilei;LI Dan;SUN Xueli;TIAN Na;LIU Fan;FU Shuai;CHEN Yukun;LIN Yuling;CHENG Chunzhen;WANG Tianchi;LAI Zhongxiong(College of Horticulture/Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺学院,园艺植物生物工程研究所,福州350002

出　　处：《应用与环境生物学报》2020年第3期590-596,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31601713);国家现代农业产业技术体系专项资金(CARS-31-15);科技创新专项基金(CXZX2018076);科技创新专项基金(CXZX2017189)资助。

摘　　要：为研究补充不同光质的LED光源对香蕉组培苗增殖和相关生理生化指标的影响,以‘天宝蕉’为材料,通过设置普通荧光+LED红光(R)、普通荧光+LED蓝光(B)、普通荧光+LED绿光(G)、普通荧光+LED白光(W)、普通荧光+LED暖白光(WW)、普通荧光+LED暖黄光(WY),对照组普通荧光7种处理,比较不同LED补光对香蕉组培苗的增殖率、相关生理指标、叶绿素含量及叶绿素荧光参数的影响.结果显示,WY处理下香蕉组培苗的增殖系数最大,B处理下香蕉苗的增殖系数最小;R处理下,植株高度最大,B处理下,植株假茎最粗,WW处理下,有最大的根长,W处理下根数、根粗、叶数、叶宽、叶长的数值最大,同时鲜重和含水量最高,壮苗系数最优.与CK相比,R、B和G处理会降低叶绿素以及类胡萝卜素含量,而补充LED白光可以提高叶绿素以及类胡萝卜素含量.此外,LED补光,尤其是R、W和G补光会引起香蕉组培苗叶片Fv/Fo、Fv/Fm和NPQ参数的显著增加和Y(Ⅱ)、ETR、和qP等参数的降低.本研究表明在增殖培养阶段补充LED暖黄光,香蕉组培苗的增殖率最高;在生根培养阶段补充LED白光,香蕉组培苗的叶绿素含量、chla/chlb比值、类胡萝卜素含量最大,同时光合效率和壮苗系数最高;结果可为香蕉组培苗增殖和生根阶段的光质选择奠定基础.This study aimed to investigate the effects of LED supplementation on the proliferation,and physiological and biochemical indexes of tissue-cultured banana plantlets.‘Tianbaojiao’bananas were subjected to seven different LED treatments:ordinary fluorescence plus LED red light(R),ordinary fluorescence plus LED blue light(B),ordinary fluorescence plus LED green light(G),ordinary fluorescence plus LED white light(W),ordinary fluorescence plus LED warm white light(WW),ordinary fluorescence plus LED warm yellow light(WY),and ordinary fluorescence(CK).Treatment effects on growth rate,physiological indexes,chlorophyll content,and chlorophyll fluorescence of banana plantlets were then compared.The multiplication coefficient of banana plantlets was found to be the highest under WY treatment and the smallest under B treatment.Plantlets were tallest under R treatment,the largest pseudostem diameter was recorded under B treatment,and the root length was greatest under WW treatment.The maximum root length,root number,root diameter,leaf number,leaf width,and leaf length were greatest under W treatment.Moreover,CK,R,B,and G treatments resulted in inhibited chlorophyll and carotenoid accumulation,while white light supplementation increased chlorophyll and carotenoid content.Plants in the W group also showed the highest fresh weight,water content,and optimum seedling coefficient.Furthermore,R and G treatments with monochromatic light decreased chlorophyll and carotenoid content,while white LED supplementation increased chlorophyll and carotenoid content.In addition,supplemental LED light,especially red,white,and green light significantly increases the parameters of Fv/Fo,Fv/Fm,and NPQ,but inhibits the Y(Ⅱ),ETR,and qP parameters.During multiplication culture processes,warm yellow light supplementation resulted in the highest plantlet growth rate.During rooting,white light LED supplementation is helpful for chlorophyll and carotenoid accumulation and provides the highest chla/chlb ratio,photosynthetic efficiency,and seedling-

关 键 词：香蕉 LED补光 增殖系数 生理生化指标 叶绿素含量 叶绿素荧光 

分 类 号：S668.1[农业科学—果树学]