花生中FAR1-5转录因子的克隆和功能分析  

Cloning and Function Analysis of FAR1-5 Transcription Factor in Peanut

作　　者：闫彩霞[1] 李春娟[1] 孙全喜[1] 张浩[1,2] 王娟[1] 苑翠玲 单世华[1] 赵小波[1] YAN Cai-xia;LI Chun-juan;SUN Quan-xi;ZHANG Hao;WANG Juan;YUAN Cui-ling;SHAN Shi-hua;ZHAO Xiao-bo(Shandong Peanut Research Institute,Qingdao 266100,China;College of Agronomy,Shandong Agricultural University,Tai'an 271018,China)

机构地区：[1]山东省花生研究所,山东青岛266100 [2]山东农业大学农学院,山东泰安271018

出　　处：《花生学报》2020年第2期16-20,共5页Journal of Peanut Science

基　　金：国家自然科学基金项目(31901506);泰山学者特聘专家(ts201712080);山东省现代农业产业技术体系花生遗传育种岗位(SDAIT-04-02);农业科研杰出人才及其创新团队培养;山东省农业良种工程项目(2017LZN033,2017LZGC003);山东省农业科学院农业科技创新工程(CXGC2016A01,CXGC2016A02)。

摘　　要：FARl是一类起源于转座子酶的转录因子,在光敏色素A(phyA)信号通路启动、植物光形态建成中具有重要作用。本试验对抗旱花生品种J11的FAR1-5基因进行了克隆,通过序列比对进化分析,表明其与野生花生Arachis duranensis,A.ipaensis相关基因具有较高相似度。干旱胁迫状态下,转基因植株抗旱性强于野生型植株,抗氧化酶活性增强,表明该转录因子可积极响应花生抗旱胁迫。研究结果可为花生抗旱育种提供新的基因资源。FAR1 is a class of transcription factors derived from mutator-like transposases,which plays an important role in the phytochrome A signaling pathway and photomorphogenesis.However,the research about abiotic stress is rare.In previous study,FAR1-5 transcription factor of peanut was found to be up-regulated under drought stress.RACE was applied to isolate and clone the full length of this gene.Based on the phylogenetic tree,peanut FAR1-5 transcription factor AhJ11-FAR1-5 was verified to have close relationship with Arachis duranensis and A.ipaensis,the diploid ancestors of cultivated peanut.Under normal conditions,no obvious phenotype difference was observed between the transgenic and wild-type seedlings.Meanwhile,the overexpression of AhJ11-FAR1-5 in peanut resulted in enhanced tolerance to drought stress,as determined by the assessment of antioxidant enzyme activities.The results indicated that AhJ11-FAR1-5 had important role in peanut responding to drought stress.This study provided gene resource for the peanut drought resistant breeding.

关 键 词：FAR1-5转录因子 花生 过表达 干旱胁迫 基因克隆 

分 类 号：S565.203.4[农业科学—作物学] Q781[生物学—分子生物学]