利用制备液相色谱系统分离中药槐花米中的芦丁  

Separation of rutin from the Chinese herb Flos Sophorae Immaturus by preparative HPLC

作　　者：彭毓敏 朱峰 周炎[2,3] 古世阳 PENG Yu-min;ZHU Feng;ZHOU Yan;GU Shi-yang(Drug Dispensing Center,Guangdong Traditional Chinese and Western Medicine Integrated Hospital,Foshan 528200,China;School of Environment and Chemical Engineering,Foshan University,Foshan 528000,China;Foshan City Engineering&Technology Research Center for Bioactive Natural Products&Functional Chemicals,Foshan University,Foshan 528000,China)

机构地区：[1]广东省中西医结合医院药物调配中心,广东佛山528200 [2]佛山科学技术学院环境与化学工程学院,广东佛山528000 [3]佛山科学技术学院佛山市活性天然产物与功能化学品工程技术研究中心,广东佛山528000

出　　处：《佛山科学技术学院学报（自然科学版）》2020年第3期60-64,共5页Journal of Foshan University(Natural Science Edition)

基　　金：广东省自然科学基金资助项目(2016A030313380);佛山市卫生和计划生育局医学科研资助项目(20190273);佛山市科技计划资助项目(2018AB003721)。

摘　　要：目的开发一种利用制备液相色谱系统从槐花米中制备高纯度芦丁的快速方法。方法槐花米粉末先用甲醇提取得到粗芦丁,粗芦丁再利用制备液相色谱系统分离纯化。结果使用SunFireRPrep C18 OBDTM柱(5μm,19 mm×250 mm),用0.2%乙酸水溶液-甲醇(45∶55,V/V)流动相等度洗脱,流速10 mL/min,检测波长360 nm,柱温为室温,进样量2.5 mL,采用核磁共振波谱技术对纯化芦丁进行鉴定。结论在该色谱条件下,可以有效从槐花米中分离制备芦丁,其核磁共振波谱图符合芦丁结构特征。Objective This work aims to develop a rapid method for the separation of rutin from Flos Sophorae Immaturus using the preparative HPLC.Methods The crude rutin was extracted from Flos Sophorae Immaturus by methanol,and then separated and purified using the preparative HPLC.Results A SunFireRPrep C18 OBDTM column(5μm,19 mm×250 mm)was used.The column was isocratic eluted with 0.2%aqueous acetic acid-methanol(45∶55,V/V).The flow rate was 10 mL/min.The detection wavelength was 360 nm.The column temperature was room temperature,and the injection volume was 2.5 mL.The purified rutin was identified by NMR spectroscopy.Conclusion Rutin can be separated effectively from Flos Sophorae Immaturus under this chromatographic condition,and its NMR spectra conform to the structural characteristics of rutin.

关 键 词：制备液相色谱 槐花米 芦丁 分离 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]