西安市景观水体中产ESBLs菌的种属分布及其耐药特征  

Species distribution and the antibiotic resistance characteristics of extension⁃spectrum lactamases(ESBLs)⁃producing bacteria in urban landscape waters in Xi′an City

作　　者：李永强 张崇淼[1] 张佩佩 梁杰 杨权奥 LI Yongqiang;ZHANG Chongmiao;ZHANG Peipei;LIANG Jie;YANG Quanao(Key Laboratory of Northwest Water Resource,Environment and Ecology,Ministry of Education,Shaanxi Key Laboratory of Environmental Engineering,Xi′an University of Architecture and Technology,Xi′an 710055)

机构地区：[1]西安建筑科技大学,西北水资源与环境生态教育部重点实验室,陕西省环境工程重点实验室,西安710055

出　　处：《环境科学学报》2020年第7期2522-2527,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.51578441)。

摘　　要：为了解城市景观水体中产超广谱β-内酰胺酶(extended-spectrum β-lactamases,ESBLs)细菌的种属分布和耐药特征,从西安市4处景观水体中分离出385株异养菌,利用双纸片协同试验筛选产ESBLs菌,并通过16S rDNA测序比对鉴定其种属.使用K-B纸片琼脂扩散法测定产ESBLs菌对氨苄西林、四环素、磺胺甲恶唑、环丙沙星和头孢唑肟的耐药表型.采用聚合酶链反应测定这些菌株是否携带β-内酰胺类耐药基因blaTEM、blaCTX-M和blaSHV以及Ⅰ类整合酶基因intI.结果表明:从城市景观水体中分离出19株产ESBLs菌,在异养菌中的占比为4.94%.这些产ESBLs菌株共计6属10种,主要为大肠埃希氏菌.它们对氨苄西林、四环素和磺胺甲恶唑普遍耐药,多重耐药菌占比为57.89%.这些产ESBLs菌株中Ⅰ类整合子检出率高达89.47%,但3种β-内酰胺酶耐药基因的检出率很低.In order to understand the species distribution and antibiotic resistance characteristics of extension⁃spectrum lactamases(ESBLs)⁃producing bacteria,385 strains of heterotrophic bacteria were isolated from four urban landscape waters in Xi′an city.Among them,ESBLs⁃producing bacteria isolates were screened by using double⁃disc synergy test.These ESBLs⁃producing bacteria were identified with 16S rDNA sequencing analysis,and the phenotypes resistant to ampicillin,tetracycline,sulfamethoxazole,ciprofloxacin and ceftizoxime were determined by K⁃B disc agar diffusion method.Polymerase chain reaction was used to determine the strains carried three majorβ⁃lactamases resistance genes blaTEM,blaCTX⁃M and blaSHV,as well as class I integrase gene intI.The results showed that 19 ESBLs⁃producing strains were isolated from the urban landscape waters,accounting for 4.94%of the heterotrophic bacteria.These ESBLs⁃producing bacteria were classified in 6 genera and 10 species,mainly Escherichia coli.The isolate ESBLs⁃producing strains were generally resistant to ampicillin,tetracycline and sulfamethoxazole,and the proportion of multiple⁃antibiotic⁃resistant bacteria was 57.89%.The detection rate of class I integron was as high as 89.47%,while the detection rates of threeβ⁃lactamases resistance genes were low among the ESBLs⁃producing isolates.

关 键 词：城市景观水体 产超广谱Β-内酰胺酶细菌 种属 多重耐药菌 Ⅰ类整合子 

分 类 号：X522[环境科学与工程—环境工程] X172