利用宏DNA条形码研究浮游动物多样性——以鸭绿江口为例  

Research on zooplankton diversity using DNA-based metabarcoding technique:a case study in the Yalvjiang Estuary

作　　者：高养春 李海涛[3] 王孝程[1] 孙艺 战爱斌 Aileen TAN Shau-Hwai[4] 李宏俊 GAO Yangchun;LI Haitao;WANG Xiaocheng;SUN Yi;ZHAN Aibin;Aileen TAN Shau⁃Hwai;LI Hongjun(National Marine Environmental Monitoring Center,Dalian 116023,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100853,China;South China Sea Environmental Monitoring Center,State Oceanic Administration,Guangzhou 510300,China;Centre for Marine and Coastal Studies,Universiti Sains Malaysia,11800,Penang,Malaysia;Guangdong Institute of Applied Biological Resources,Guangzhou 510260,China)

机构地区：[1]国家海洋环境监测中心,大连116023 [2]中国科学院生态环境研究中心,北京100853 [3]国家海洋局南海环境监测中心,广州510300 [4]Centre for Marine and Coastal Studies,Universiti Sains Malaysia(USM),11800,Penang,Malaysia [5]广东省生物资源应用研究所,广州510260

出　　处：《生态学报》2020年第11期3822-3832,共11页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31572595);中国—东盟海上合作基金(全球变化背景下南中国海近海生态系统监测与保护管理示范);业务化监测项目(鸭绿江口生态综合监测示范)。

摘　　要：为评价宏DNA条形码技术在我国海洋生物多样性监测中的应用潜力,采集了22份鸭绿江口浮游动物样品,分别利用宏条形码分子鉴定和形态鉴定方法对优势类群桡足类进行多样性的比较研究。结果显示:(1)利用宏条形码分子鉴定方法共鉴定出4目23科32属229个操作分类单元(Operational Taxonomic Units, OTUs),形态方法共鉴定出3目5科5属6种;同时,利用形态鉴定得到的分类阶元多数(目:100%、科:80%、属:80%)能用宏条形码分子鉴定方法鉴定出来,而宏条形码分子鉴定方法鉴定得到的分类阶元多数(目:25%、科:83%、属:88%)却未能用形态鉴定出来,表明宏条形码分子鉴定方法在鉴定物种丰富度方面具有明显优势。(2)利用宏条形码分子鉴定与形态鉴定桡足类的多样性指数呈显著的一致性(r=0.524,P=0.024),表明宏条形码鉴定方法与形态方法在评价物种多样性方面具有较好的可比性。本研究表明宏条形码分子鉴定方法在我国海洋浮游动物业务化监测中具有较高的应用潜力。In order to assess the power of DNA-based metabarcoding in the monitoring of marine biodiversity, we ultilized both metabarcoding technique and morphological method to compare communtiy diverstiy of copepods, which were collected from 22 sites along the Yalvjiang Estuary of China. The results showed that:(1) a total of four order, 23 family, 32 genus and 229 operational taxonomic units(OTUs) were detected through the metabarcoding technique, whereas only three order, five familiy, five genus and six species were identified based on the morphological method. In addition, most taxonomic categories(100% order, 80% family, and 80% genus) identified based on the morphological method could be also detected using the metabarcoding technique. Reversly, however most taxonomic categories(25% order, 83% family and 88% genus) detected using the the metbarcoding technique could not be identified based on the morphological method. The results suggested that the metabarcoding technique was more powerful than the morphological method in the assessment of copepod diversity.(2) The variation of diversity index based on the metabarcoding method was significantly consistent with the morphological method(r=0.542, P=0.024), suggesting good comparability between the two methods. Our results indicated that the DNA-based metabarcoding technique had high application potential in efficient assessment of marine biodivesity.

关 键 词：浮游动物 群落结构 宏DNA条形码 18S 鸭绿江口 

分 类 号：Q958.8[生物学—动物学]