甘肃野生草地早熟禾种质资源无融合生殖鉴定分析  

Identification of apomictic characteristics of wild Kentucky bluegrass germplasm resources in Gansu

作　　者：刘燕 张金青 牛奎举 董文科[1] 马晖玲[1] 李玉珠[1] LIU Yan;ZHANG Jin-qing;MA Hui-ling;NIU Kui-ju;DONG Wen-ke(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S .Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2020年第3期84-89,共6页Grassland and Turf

基　　金：国家自然基金项目“甘肃野生草地早熟禾无融合生殖胚发生的分子遗传机理研究”(31760699)。

摘　　要：以甘肃省8个地区的野生草地早熟禾和商用品种‘午夜2号’为试验材料,通过纸上萌发法统计多胚苗率,利用石蜡切片技术统计无融合生殖率,并探究两者之间的相关性。结果表明:参试草地早熟禾材料的多胚苗率为0~15.29%,其中最高的是陇南,最低的是甘南,二者差异显著;无融合生殖率最高的是陇南,可达到71.04%,最低的是甘南,仅为11.2%,其他种质的无融合生殖率差距比较小,为32%~58%;相关性分析表明,多胚苗率与无融合生殖率存在显著相关性(R2=0.96),该研究结果可为草地早熟禾无融合生殖材料筛选、种植栽培及其遗传育种提供一定的技术参考。Eight wild Kentucky bluegrass populations from different areas in Gansu and one commercial cultivar‘MidnightⅡ’were used to study their apomictic frequency and poly-embryo rate by using Paraffin sectioning technique and paper germination method.The results indicated that the poly-embryo rate ranged from 0%to 15.29%,the highest was from Longnan and the lowest was from Gannan.The highest apomictic occurrence frequency was from Longnan(71.04%),and the lowest was from Gannan(11.2%),and the rest ranged from 32%to 58%.Correlation analysis showed that there was a significant correlation between apomictic frequency and poly-embryo rate(R2=0.96).breeding of Kentucky bluegrass.

关 键 词：草地早熟禾 石蜡切片技术 无融合生殖率 多胚苗率 

分 类 号：S688.4[农业科学—观赏园艺]