不同分子量醋柴胡多糖对Raw264.7巨噬细胞抗炎免疫活性的影响  

Study on Anti-inflammatory and Immunomodulatory Activity of Vinegar Bupleuri Radix Polysaccharides with Different Molecular Weight on Raw264.7 Macrophage

作　　者：李苹 卢柏林 邓时贵 赵瑞芝 赵亚 LI Ping;LU Bolin;DENG Shigui;ZHAO Ruizhi;ZHAO Ya(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;School of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学第二附属医院,广州510006 [2]广州中医药大学中药学院,广州510006

出　　处：《中国现代应用药学》2020年第10期1153-1156,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81703798,81573612)。

摘　　要：目的探讨不同分子量醋柴胡多糖对Raw264.7小鼠巨噬细胞抗炎免疫活性的影响。方法采用截留不同分子量的透析袋将醋柴胡多糖进行不同分子量分离;采用MTT检测不同分子量醋柴胡多糖对Raw264.7小鼠巨噬细胞活力的影响;采用中性红吞噬实验检测多糖对细胞吞噬活性的影响;采用Griess法检测不同分子量醋柴胡多糖对LPS刺激Raw264.7炎症细胞模型NO释放的影响。结果分离得到<3.5,3.5~50,50~100,100~300,300~1 000,>1 000 kDa 6个不同分子量段的醋柴胡多糖;各分子量段醋柴胡多糖在1.95~31.25μg·mL^-1对巨噬细胞无毒性;除<3.5 kDa分子量段外,其他分子量段醋柴胡多糖能显著促进巨噬细胞吞噬活性,且分子量>100 kDa醋柴胡多糖活性更显著;各分子量段醋柴胡多糖均能显著抑制LPS刺激Raw264.7炎症细胞NO的释放。结论分子量是影响醋柴胡多糖抗炎免疫活性差异的重要因素。OBJECTIVE To explore the effect of vinegar Bupleuri Radix polysaccharides with different molecular weight on the anti-inflammatory and immunomodulatory activity of Raw264.7 macrophage.METHODS Vinegar Bupleuri Radix polysaccharides with different molecular weight were separated by dialysis bags.MTT assay was used to determine the effect of vinegar Bupleuri Radix polysaccharides with different molecular weight on cell viability of Raw264.7 macrophage;neutral red phagocytosis assay was used to determine the effect of polysaccharides on cell phagocytosis;Grie ss method was used to detect their effect on the release of NO of Raw264.7 inflammatory cell model stimulated by LPS.RESULTS Six different molecular weight of vinegar Bupleuri Radix polysaccharides(<3.5,3.5-50,50-100,100-300,300-1000,>1000 kDa)were separated,and they had no cell toxicity to Raw264.7 macrophage in the range of 1.95-31.25μg·mL^-1.In addition to vinegar Bupleuri Radix polysaccharides with molecular weight<3.5 kDa,others significantly promoted the phagocytic activity of macrophages,and the activity of polysaccharides with molecular weights>100 kDa were more significant.All molecular weight segments of vinegar Bupleuri Radix polysaccharides significantly inhibited the release of NO in Raw264.7 inflammatory cells stimulated by LPS.CONCLUSION Molecular weight is an important factor affecting the activity of anti-inflammatory and immunomodulatory.

关 键 词：醋柴胡多糖 分子量 RAW264.7巨噬细胞 抗炎 免疫 

分 类 号：R285.5[医药卫生—中药学]