基于Nrf2/HO-1通路探讨石参有效成分对鱼藤酮诱导的帕金森病细胞模型的保护作用  

Neuroprotection of Uraria crinite in Parkinson′s disease cell models based on the Nrf2/HO⁃1 pathway

作　　者：赵燕芬[1] 吴伟斌[1] 梁结斐 梁少明[1] 刘文华[2] ZHAO Yanfen;WU Weibin;LIANG Jiefei;LIANG Shaoming;LIU Wenhua(Zhaoqing Medical College,Zhaoq⁃ing 526020,China;不详)

机构地区：[1]肇庆医学高等专科学校药理教研室,广东肇庆526020 [2]肇庆学院生命科学学院生物科学教研室,广东肇庆526020

出　　处：《实用医学杂志》2020年第13期1745-1749,共5页The Journal of Practical Medicine

基　　金：广东省普通高校青年创新人才类项目(编号:2018GkQNCX020);肇庆市科技创新指导类项目(编号:201804031102)。

摘　　要：目的探讨石参提取物对鱼藤酮诱导的帕金森病体外模型的保护作用。方法以鱼藤酮诱导人神经母细胞瘤细胞SH-SY5Y细胞建立帕金森病体外模型,通过Cell Counting Kit-8法(CCK-8)研究药物的保护作用、2,7-二氯四氟乙烷双乙酸盐(DCFH-DA)法评估细胞内活性氧(ROS)、化学显色法测定细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及谷胱甘肽过氧化物酶(GPx)的含量、Western blot法检测核因子E2相关因子2(Nrf2)及血红素加氧酶-1(HO-1)蛋白的表达量。结果石参提取物能有效对抗鱼藤酮引起的细胞损伤及细胞内活性氧升高,且能升高SOD、GSH及GPx含量同时降低MDA的含量,还能同时上调Nrf2及HO-1的蛋白表达量。结论石参提取物保护鱼藤酮诱导的SH-SY5Y细胞帕金森病模型的作用可能与增强Nrf2/HO-1通路功能有关。Objective To investigate the protective effect of the Uraria crinite extract on rotenone⁃induced Parkinson′s disease(PD)model in vitro.Methods The SH⁃SY5Y cells were induced to build a Parkinson′s disease model by rosterone,and the protective effect of drugs was studied by Cell Counting Kit⁃8(CCK⁃8),the intracellular reactive oxygen species(ROS)was evaluated by 2,7⁃dichlorotetrafluoroethane diacetate(DCFH⁃DA),the intra⁃cellular contents of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH)and glutathione peroxidase(GPx)were determined by chemical coloration kit,and the expression level of nuclear factor E2⁃related factor 2(Nrf2)and heme oxygenase⁃1(HO⁃1)protein was detected by Western blot.Results The extract of Uraria crinite could effectively relieve the damage caused by rotenone,and reduced the intracellular ROS,increased the content of SOD,GSH and GPx,and reduced the content of MDA,and up⁃regulated the expression levels of Nrf2 and HO⁃1 protein.Conclusion The protective effect of Uraria crinite extracts on rotenone⁃induced cell model of PD may be related to the enhancement of Nrf2/HO⁃1 pathway function.

关 键 词：石参 帕金森病 鱼藤酮 抗氧化 

分 类 号：R965.1[医药卫生—药理学]