干旱胁迫及复水对花叶芦竹生理特性的影响  

Effects of drought stress and rewatering on physiological characteristics of Arundo donax var.versicolor

作　　者：许令明 曹昀[1] 汤思文 陆远鸿 罗姗姗 马银山[2] XU Lingming;CAO Yun;TANG Siwen;LU Yuanhong;LUO Shanshan;MA Yinshan(School of Geography and Environment, Jiangxi Normal University/Key Laboratory of Poyang Lake Wetland and Watershed Research Ministry of Education, Jiangxi Normal University, 330022, Nanchang, China;College of Agriculture and Biotechnology, Hexi University, 734000, Zhangye, Gansu, China)

机构地区：[1]江西师范大学地理与环境学院/鄱阳湖湿地与流域研究教育部重点实验室,南昌330022 [2]河西学院农业与生物技术学院,甘肃张掖734000

出　　处：《中国水土保持科学》2020年第3期59-66,共8页Science of Soil and Water Conservation

基　　金：国家自然科学基金“鄱阳湖洲滩典型湿地植物的水位生态幅研究”(41361017);“环境条件和放牧干扰对肃南草地物种多样性影响机制的研究”(31660161);江西省自然科学基金“鄱阳湖灰化苔草季节性变化的水文驱动机制研究”(20181BAB203021)。

摘　　要：通过在干旱胁迫与复水条件下对花叶芦竹生理特征的影响研究,以期为湿地园林建设中引种栽培及管理提供借鉴。采用室内盆栽法,设置9个水分梯度[CK:(530.5)%(土壤水分饱和)、C1:(500.5)%、C2:(450.5)%、C3:(400.5)%、C4:(350.5)%、C5:(300.5)%、C6:(200.5)%、C7:(100.5)%、C8:(50.5)%],研究干旱胁迫及复水对花叶芦竹生理特性的影响。结果表明:1)随胁迫时间延长,花叶芦竹叶片叶绿素质量分数呈下降趋势,试验后期随胁迫程度加深其质量分数减小;随胁迫时间延长类胡萝卜素质量分数在土壤水分含量40%~50%之间呈先降后升趋势,对照与其余处理组呈下降趋势;2)随胁迫时间延长,SOD活性呈先升后降趋势,POD活性呈先降后升趋势,CAT活性呈上升趋势;随胁迫程度加深,SOD活性呈上升趋势,而POD、CAT活性均呈先升后降趋势;3)随胁迫时间延长,可溶性蛋白、MDA含量均呈先升后降趋势,均随着胁迫程度的加深而增加;4)复水后,花叶芦竹叶片光合色素含量上升,在40%~50%处理均高于对照;抗氧化酶活性均下降,SOD活性与对照差异不明显,POD活性与对照差异显著,CAT活性与对照差异极显著;可溶性蛋白、MDA含量下降;根系活力显著高于复水前。综上,干旱胁迫下,花叶芦竹叶片光合色素合成受到抑制,但能通过协调抗氧化酶清除自由基,及积累可溶性蛋白应对氧化伤害;复水后,各项指标均能恢复到对照水平,表现出一定耐旱性。[Background]Moisture is the main component of plants,affecting the growth,development,metabolism and geographical distribution of plants.Recently,plant physiological ecology research mainly focuses on drought stress,photosynthesis,chlorophyll fluorescence,antioxidant system and osmotic adjustment substances.The effects of drought stress and rewatering on the physiological characteristics of Arundo donax var.versicolor were studied in order to provide reference for the introduction,cultivation and management of wetland gardens and to provide more choices for the use of wetland plants for soil and water conservation in wetland parks.[Methods]Taking the A.donax var.versicolor seedlings as experimental materials,the effects of drought stress and rewatering on the physiological characteristics of A.donax var.versicolor were studied.The indoor pot experiment was conducted to set 9 water gradients[CK:(530.5)%(Soil water saturation),C1:(500.5)%,C2:(450.5)%,C3:(400.5)%,C4:(350.5)%,C5:(300.5)%,C6:(200.5)%,C7:(100.5)%,C8:(50.5)%].The chlorophyll content,carotenoid content,antioxidant enzyme activity,soluble protein content,malondialdehyde content,and root activity of A.donax var.versicolor were determined,and the effects of different stress treatments on its physiology were analyzed by SPSS 21.0、Microsoft-Excel 2010 and One-wayANOVA.[Results]1)With the prolongation of stress time,the chlorophyll content of A.donax var.versicolor leaves decreased with the degree of stress deepening,the content of carotenoids decreased first and then increased during the 40%-50%soil moisture content with the prolongation of stress time,while the control and other treatment groups showed a decreased trend.2)With the prolongation of stress time,the activity of SOD increased first and then decreased,the activity of POD decreased first and then increased,and the activity of CAT increased.With the degree of stress deepening,the activity of SOD increased,while the activities of POD and CAT increased first and then decreased.3)With the prolongatio

关 键 词：花叶芦竹 干旱胁迫 复水 光合色素 抗氧化酶 

分 类 号：S157[农业科学—土壤学]