宁夏水稻种质资源主要抗稻瘟病基因的鉴定和评价  

Identification and Evaluation of Main Rice Blast Resistance Genes in Rice Germplasm Resources of Ningxia

作　　者：张银霞[1] 沈文娟[2] 韩雪琴 张振海[3] 田蕾[1] 杨淑琴[1] 罗成科 李培富[1] ZHANG Yinxia;SHEN Wenjuan;HAN Xueqin;ZHANG Zhenhai;TIAN Lei;YANG Shuqin;LUO Chengke;LI Peifu(College of Agriculture,Ningxia University,Yinchuan 750021,China;Ningxia Hui Autonomous Region Original Seed Farm,Yinchuan 750200,China;Institute of Crop Sciences,Ningxia Academy of Agricultural and Forestry Sciences,Yongning Ningxia 750105,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏回族自治区原种场,银川750200 [3]宁夏农林科学院农作物研究所,宁夏永宁750105

出　　处：《西北农业学报》2020年第7期1009-1019,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：宁夏自然科学基金(2018AAC03009);宁夏农业育种专项水稻新品种选育项目(2018NYYZ0302);水稻优异种质资源鉴定与利用(2018NYYZ0301)。

摘　　要：针对宁夏地区水稻抗稻瘟病主要基因尚不清楚的现状,采用已克隆抗稻瘟病基因的功能标记对143份宁夏水稻种质资源进行抗稻瘟病基因的分子扫描。同时,利用宁夏地区致病性强且致病力频率高的10个稻瘟病菌菌株混合悬浮液进行人工接种与自然诱发相结合的方法鉴定其抗病性。进而开展稻瘟病抗性性状与抗性基因的关联分析。结果表明:在宁夏水稻种质资源中表现主要的抗病基因有Pikm、Pi9、Pi2、Pi5和Pid2。在此基础上,对主要抗病基因在宁夏水稻品种中的应用进行评价,发现Pikm、Pi9、Pi5、和Pid2基因在宁夏水稻种质资源中的分布频率比较低,分别为0.56、0.35、0.30、和0.11。所以这些基因是今后改良宁夏水稻稻瘟病抗性的主要抗病基因。In order to detect the main genes of rice blast resistance in Ningxia,the functional markers of cloned rice blast resistance genes were used to identify the genotype of 143 Ningxia rice germplasm resources in this study,meanwhile,the mixed suspension of 10 Magnaporthe oryzae strains with strong pathogenicity and high virulence frequency in Ningxia was used to conduct artificial inoculation and natural induction for identifying the disease resistance.Furthermore,the association analysis between rice blast resistance traits and resistance genes was carried out.The results showed that the main rice blast resistance genes in rice germplasm resources of Ningxia were Pikm,Pi9,Pi2,Pi5 and Pid2.Based on the results above,the application of these main blast resistance genes in rice varieties of Ningxia was evaluated.In conclusion,the distribution frequency of Pikm,Pi9,Pi5,and Pid2 genes are relatively low,which are 0.56,0.35,0.30,and 0.11,respectively,and it is the main genes for increasing rice blast resistance of Ningxia.

关 键 词：水稻 主要抗稻瘟病基因 鉴定 

分 类 号：S511.2[农业科学—作物学]