基于SLAF-seq技术的山西省地方梨品种的SNP分析  

SNP Analysis of Local Pear Germplasm Resources in Shanxi Province Based on SLAF-seq Technology

作　　者：白牡丹[1] 郝国伟[1] 张晓伟[1] 杨盛[1] 郭黄萍[1] BAI Mudan;HAO Guowei;ZHANG Xiaowei;YANG Sheng;GUO Huangping(Pomology Institute,Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Germplasm Improvement and Utilization in Pomology,Taiyuan 030031,China)

机构地区：[1]山西省农业科学院果树研究所/果树种质创制与利用山西省重点实验室,太原030031

出　　处：《西北农业学报》2020年第7期1020-1027,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-28-28);山西省农业科学院生物育种工程项目(17yzgc036),山西省农业科学院应用基础研究计划项目(YCX2020YQ09)。

摘　　要：利用简化基因组测序技术SLAF-seq测序对30个山西省地方梨品种群体结构与亲缘关系进行分析,旨在为梨育种及其资源利用提供参考。通过序列分析,获得603177个SLAF标签,其中多态性的SLAF标签302703个,共得到2140079个群体SNP。利用这些SNP标签分析可得30个梨品种可以分为两大类。通过构建进化树发现,‘金梨’与‘大黄梨’虽田间性状比较相似,但在进化树上显示两者亲缘关系较远,可以断定二者为不同梨品种。The population structure and phylogenetic relationships among 30 local pear varieties in Shanxi province were analyzed by using specific-locus amplified fragment sequencing(SLAF-seq),which will provide reference for pear breeding and utilization.Based on bioinformatics analysis,603177 SLAFs including 302703 polymorphic SLAFs were obtained.A total of 2140079 single nucleotide polymorphisms(SNPs)was obtained by sequence analysis.Thirty pear varieties analyzed by SNP dataset could be divided into two categories.Although the characters of‘Jinli’and‘Dahuangli’pear were similar in the field,but they were far from each other in the phylogenetic tree,so it belonged to different pear varieties.

关 键 词：梨 SLAF-seq SNP 群体结构 进化树 

分 类 号：S661.2[农业科学—果树学]