Na2CO3胁迫对山柿幼苗生长和生理特性的影响  

Effect of Na2CO3 Stress on Growth and Physiological Properties of Diospyros japonica Seedlings

作　　者：殷梦竹 颜玉娟[1] 颜立红[2] YIN Meng-zhu;YAN Yu-juan;YAN Li-hong(Central South University of Forestry and Technology,Changsha 410004,China;Hunan Forest Botanical Garden,Changsha 410116,China)

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]湖南省森林植物园,湖南长沙410116

出　　处：《浙江林业科技》2020年第2期23-29,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：湖南省林业厅科技创新项目(XLK201756);中央财政林业科技推广项目﹝2019(XT001)﹞;国家林业局“十三五”重点学科项目(﹝2015﹞44);湖南省教育厅“十二五”重点学科项目(2011-76);湖南高校一流本科专业(园林专业)(湘教通〔2019〕138号)。

摘　　要：为揭示山柿Diospyros japonica对盐环境的适应能力,为山柿的推广应用提供理论依据,以2年生山柿幼苗为材料,采用盆栽实验法,设置空白对照组(CK),1 g·L^-1,2 g·L^-1,4 g·L^-1,6 g·L^-1和8 g·L^-1 Na2CO3胁迫处理,分别于处理后8 d,16 d,24 d和32 d时测定其生长和生理指标,研究山柿的耐盐性。结果表明,胁迫0~32 d,随着胁迫浓度的上升,株高和地径增长量均呈先上升后下降的趋势,叶片相对含水量则有明显下降,吸水能力受到影响;随着胁迫时间的延长和胁迫浓度的上升,丙二醛含量有所上升,过氧化物酶和超氧化物歧化酶活性均有一定程度的上升以减轻氧化损伤;在整个实验期间,各胁迫处理的山柿能够大量积累渗透调节物质可溶性蛋白和可溶性糖以维持其渗透势。研究表明,山柿在1~4 g·L^-1 Na2CO3胁迫下具有一定的抗性,能够自我调节适应胁迫环境;而在较高浓度即6 g·L^-1和8 g·L^-1胁迫下虽然具备一定的调节能力,但依然会受到一定程度的损伤。In May 2019,2-year Diospyros japonica seedlings were selected from Hunan Forest Botanical Garden for ball transplanting in pot.Slow rooting was carried out for 2 weeks.Experiment was implemented on Na2CO3 stress on these seedlings with 1 g/L,2 g/L,4 g/L,6 g/L and 8 g/L,and water as control.On the 8th,16th,24th and 32nd day,the growth and physiological properties of seedlings were determined to study its salt tolerance.The results showed that height and ground diameter growth of treated seedlings increased first and then decreased with the increase of Na2CO3 concentration,the relative water content in leaves decreased obviously.With duration of stress and the increase of Na2CO3 concentration,MDA content of treated seedlings increased;the activities of peroxidase and superoxide dismutase both increased to reduce oxidative damage.Treated seedlings could accumulate a large amount of osmoregulation substances(soluble protein and soluble sugar)to keep its osmotic potential.The conclusion revealed that D.japonica seedlings treated by 1-4 g/L Na2CO3 had resistance,but that by 6g/L and 8g/L got damaged.

关 键 词：山柿幼苗 NA2CO3胁迫 生长指标 抗氧化酶 渗透调节物质 

分 类 号：S718.43[农业科学—林学]