52例多发性骨髓瘤患者细胞免疫表型分析  

Analysis on cellular immunophenotype of 52 cases of multiple myeloma

作　　者：苏荣[1] 黎观霞 吕微风 杨延斌[3] 唐少俊 SU Rong;LI Guanxia;LYU Weifeng;YANG Yanbin;TANG Shaojun(Department of Clinical Laboratory,Foshan Municipal Hospital of Traditional Chinese Medicine,Foshan,Guangdong 528000,China;College of Inspection,Guangdong Medical University,Zhanjiang,Guangdong 524000,China;Statistics Room,Foshan Municipal Hospital of Traditional Chinese Medicine,Foshan,Guangdong 528000,China;Computer Network Center,Foshan Municipal Hospital of Traditional Chinese Medicine,Foshan,Guangdong 528000,China)

机构地区：[1]广东省佛山市中医院检验科,广东佛山528000 [2]广东医科大学检验学院,广东湛江524000 [3]广东省佛山市中医院统计室,广东佛山528000 [4]广东省佛山市中医院计算机网络中心,广东佛山528000

出　　处：《国际检验医学杂志》2020年第14期1690-1693,1698,共5页International Journal of Laboratory Medicine

基　　金：广东省佛山市医学类科技攻关项目(2018AB002671)。

摘　　要：目的探讨52例多发性骨髓瘤患者细胞免疫表型的特点。方法采用流式细胞仪,以CD45/SSC配合CD38^+/CD138^+联合设门,对52例多发性骨髓瘤患者的骨髓细胞进行CD38、CD138、CD19、CD56、CD117、CD200标记检测,并进行骨髓细胞形态学检查。结果流式细胞术检测多发性骨髓瘤患者骨髓细胞中骨髓瘤细胞比例为0.2%~52.9%,而在细胞形态学检查中,发现骨髓瘤细胞比例为2.0%~85.5%,但两种方法检测结果之间具有良好的相关性(R^2=0.6864,P<0.05)。多发性骨髓瘤细胞各免疫表型阳性率如下:CD38为100.00%(52/52),CD138为100.00%(52/52),CD19为9.62%(5/52),CD56为80.77%(42/52),CD117为40.38%(21/52),CD200为26.92%(14/52)。其中CD56在两种轻链型和不同类型免疫球蛋白中的表达差异均有统计学意义(P<0.05)。结论κ链型患者CD56的阳性率比λ链型低,CD56的阳性率在IgM型中最低。在筛选细胞方面,流式细胞术比细胞形态学更灵敏,但细胞形态学检查的骨髓瘤细胞结果比流式细胞术结果更准确。Objective To discuss the characteristics of cellular immunophenotype in 52 cases of multiple myeloma(MM).Methods The flow cytometer was used to conduct the label detection of CD38,CD138,CD19,CD56,CD117 and CD200 in bone marrow of 52 cases of MM with CD45/SSC coordinated with CD38^+/CD138^+ as combined gating.The marrow cell morphology examination was performed.Results The flow cytometry detection showed that the proportion of myeloma cells in MM patients was 0.2%-52.9%,while which in cell morphology examination was 2.0%-85.5%,however,there was a good correlation between the detection results by these two methods(R 2=0.6864,P<0.05).Moreover,the positive rates of various immunophenotype in MM were as follows 100.00%(52/52)for CD38,100.00%(52/52)for CD138,9.62%(5/52)for CD19,80.77%(42/52)for CD56,40.38%(21/52)for CD117 and 26.92%(14/52)for CD200 respectively.Among them,there was statistically significant differences in the expression of CD56 between the two light chains and different immunoglobulin types(P<0.05).Conclusion The CD56 positive rate in the patients with kappa light chain types is lower than that in the patients with lambda light chain types.The positive rate of CD56 is the lowest in the patients with IgM type.In screening cells,the flow cytometry is more sensitive than the cell morphology,but the results of myeloma cells detected by the cell morphology examination is more accurate those than by the flow cytometry.

关 键 词：多发性骨髓瘤 流式细胞术 免疫表型 

分 类 号：R446.63[医药卫生—诊断学] R733.3[医药卫生—临床医学]