蛋白核小球藻光合荧光参数对Cu^2+毒性响应特性研究  

Response of Photosynthetic Fluorescence Parameters of Chlorella Pyrenoidosa to Cu^2+Toxicity

作　　者：胡丽 殷高方 赵南京 陈敏 甘婷婷[2] Hu Li;Yin Gaofang;Zhao Nanjing;Chen Min;Gan Tingting(Physics and Materials Engineering College,Hefei Normal University,Hefei 230061,China;Key Laboratory of Environmental Optics and Technology,Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Science,Hefei Anhui230031,China;University of Science and Technology of China,Hefei Anhui230026,China)

机构地区：[1]合肥师范学院物理与材料工程学院,安徽合肥230061 [2]中国科学院安徽光学精密机械研究所中科院环境光学与技术重点实验室,安徽合肥230031 [3]中国科学技术大学,安徽合肥230026

出　　处：《光学学报》2020年第12期146-152,共7页Acta Optica Sinica

基　　金：国家重点研发计划(2016YFC1400600);安徽省杰出青年科学基金项目(1908085J23);中科院仪器设备功能开发技术创新项目(Y93H3g1251);中国科学院环境光学与技术重点实验室开放基金项目(2005DP173065-2017-01);中国科学院院长基金项目(YZJJZX202006)

摘　　要：以蛋白核小球藻为受试对象,以藻类光合荧光参数为毒性评价指标,研究Cu^2+毒性作用下多个光合荧光参数的响应规律。结果显示:Cu^2+对光合荧光参数F0、Fm、Fv、Fv/Fm、Yield、rP、JVPⅡ、α和Ek抑制效应显著,其中Yield、rP、α和Fv/Fm在24 h内体现出稳定的抑制效应,可作为24 h分析Cu^2+毒性的评价指标;Yield、rP、α和Fv/Fm抑制程度对Cu^2+浓度具有良好的剂量效应关系,logistic函数拟合相关系数R2分别为0.9989,0.9992,0.9991,0.9977,由此得到EC50-24h值分别为61.05,66.31,69.41,99.61μmol·L^-1。因此,参数Yield、rP、α的拟合相关系数优于常规参数Fv/Fm,其对应的EC50-24h分别降低了38.7%,33.45%,30.3%。研究结论可为基于藻类光合抑制效应的生物毒性测试指标选择提供参考。Herein,Chlorella pyrenoidosa was taken as the subject,and photosynthetic fluorescence parameters of algae were used as toxicity evaluation indexes to study the response law of multiple photosynthetic fluorescence parameters under Cu^2+toxicity.The results are as follows:the inhibition effect of F0,Fm,Fv,Fv/Fm,Yield,rP,JVPⅡ,αand Ek on Cu^2+toxicity is significant,and yield,rP,αand Fv/Fm could be used as the evaluation indexes for Cu^2+toxicity analysis at 24 h;the inhibitory effect of Yield,rP,αand Fv/Fm has a good dose-effect relationship with Cu^2+toxicity.The fitting correlation coefficient R2 of the logistic function is 0.9989,0.9992,0.9991 and 0.9977,and the resulting EC50-24h is 61.05,66.31,69.41 and 99.61μmol·L^-1,respectively.The parameters Yield,rP andαhave higher fitting correlation coefficients than the conventional parameter Fv/Fm,and their EC50-24h values decrease by 38.7%,33.45%and 30.3%,respectively.The experimental results could provide reference for the detection of biological toxicity based on photosynthetic inhibition effect of algae.

关 键 词：生物光学 CU^2+ 蛋白核小球藻 光合荧光参数 毒性响应 

分 类 号：X832[环境科学与工程—环境工程]