巴特日-7对肠炎大鼠及其血清TNF-α、IL-1β、IL-10水平的影响  

Effects of Bateri⁃7 on the enteritis rats and their serum levels of TNF⁃α,IL⁃1β,and IL⁃10

作　　者：李淑艳[1] 乌云参丹 刘燕 白玉霞[1] 李淑红 王玉华 LI Shu⁃yan;Wu⁃yun⁃can⁃dan;LIU Yan;BAI Yu⁃xia;LI Shu⁃hong;WANG Yu⁃hua(College of Mongolian Medicine and Pharmacy,Inner Mongolian University for Nationalities,Tongliao 028000,China;College of Nursing,Inner Mongolian University for Nationalities,Tongliao 028000,China;College of Pharmacy,Inner Mongolian Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学护理学院,内蒙古通辽028000 [3]内蒙古医科大学药学院,内蒙古呼和浩特010110

出　　处：《中成药》2020年第7期1748-1753,共6页Chinese Traditional Patent Medicine

基　　金：内蒙古自治区科技创新引导项目(KCBJ2018030);内蒙古自治区自然科学基金项目(2017MS0882);内蒙古自治区卫生计生科研计划项目(201703130);内蒙古自治区科技计划项目子课题(KJJH2017007);内蒙古民族大学博士科研启动基金项目(BS479)。

摘　　要：目的探究巴特日-7对肠炎大鼠及其血清TNF-α、IL-1β、IL-10水平的影响。方法采用葡聚糖硫酸钠构建肠炎大鼠模型,90只SD大鼠随机分为正常对照组、模型组、阳性对照组、巴特日-7水提物组(低、中、高剂量),巴特日-7有效部位组(低、中、高剂量),肠炎大鼠造模第4天开始灌胃给药,每天1次,连续7 d。检测各组大鼠体质量、24 h的摄食量、疾病活动指数、结肠湿/干质量比,ELISA检测血清TNF-α、IL-1β、IL-10水平,HE染色观察结肠组织病理学改变。结果与模型组比较,巴特日-7水提物、巴特日-7有效部位大鼠体质量、24 h摄食量增加,疾病活动指数、结肠湿/干质量比降低,TNF-ɑ、IL-1β水平降低,IL-10水平增加(P<0.05,P<0.01),结肠组织病理学改善。结论巴特日-7能改善肠炎大鼠的症状,降低TNF-ɑ、IL-1β水平,升高IL-10水平。AIM To investigate the effects of Bateri⁃7 on the enteritis rats and their serum levels of TNF⁃α,IL⁃1β,and IL⁃10.METHODS Glucan sodium sulfate was used to induce a rat model of enteritis.Ninety SD rats were randomly divided into normal control group,model group,positive control group,Bateri⁃7 water extract groups(at low dose,medium dose and high dose,respectively),Bateri⁃7 differential extract groups(at low dose,medium dose and high dose,respectively).Enteritis rats were intragastrically administered with different agents once daily for 7 consecutive days from the 4th day after the modelling.The rats were subjected to measurement of body mass,food intake,disease activity index(DAI)and colonic wet/dry weight ratio,the determination of serum TNF⁃α,IL⁃1βand IL⁃10 levels by ELISA,and observation of colonal histopathological changes by HE staining.RESULTS Compared with the model group,groups intervened with Bateri⁃7 water extract,or Bateri⁃7 differential extract had improved body bass and the food intake at 24 h,reduced index levels revealing disease activity and colon wet/dry weight ratio,decreased TNF⁃ɑand IL⁃1βlevels,increased IL⁃10 levels(P<0.05,P<0.01),and improved colon histopathology.CONCLUSION Bateri⁃7 can improve the symptoms of enteritis rats,reduce their levels of TNF⁃ɑand IL⁃1β,and increase their IL⁃10 level.

关 键 词：巴特日-7 肠炎 TNF-ɑ IL-1Β IL-10 

分 类 号：R285.5[医药卫生—中药学]