藿苓益智方对血管性痴呆大鼠VEGF及海马氧化损伤的影响  

Effect of Huoling Yizhi Recipe on vascular endothelial growth factor and oxidative damage of hippocampus in vascular dementia rats

作　　者：吴鹏[1] 邢俊娥[1] 邢远[1] 窦维华[1] 黄立武[1] WU Peng;XING Jun-e;XING Yuan;DOU Wei-hua;HUANG Li-wu(The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning Guangxi 53000)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530001

出　　处：《世界中西医结合杂志》2020年第6期1060-1063,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(81874453);广西中医基础研究重点实验室开放项目(17-259-49-08)。

摘　　要：目的观察藿苓益智方对血管性痴呆(VD)大鼠认知功能、血管内皮生长因子(VEGF)表达及海马组织中氧化损伤的影响。方法将80只健康雄性SPF级SD大鼠随机分为假手术组、模型组、西药组、中药组,每组20只。VD模型的制备参照最经典的2-VO法建立,假手术组大鼠只进行相应的手术步骤,分离暴露双侧颈总动脉,但不进行结扎及阻断其血流供应。造模结束后西药组给予尼莫地平片灌胃,中药组给予藿苓益智方灌胃,假手术组、模型组给予等剂量生理盐水灌胃。各组采用Morris水迷宫实验来检测大鼠逃避潜伏期时间,采用免疫组化法检测大鼠VEGF表达,同时检测海马组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果(1)Morriss水迷宫实验:模型组、西药组、中药组造模后未用药前(治疗前)逃避潜伏期时间与假手术组比较明显延长,差异有统计学意义(P<0.05)。假手术组和模型组本组治疗前与用药后(治疗后)逃避潜伏期时间比较无明显变化,差异无统计学意义(P>0.05);中药组及西药组治疗后逃避潜伏期时间与本组治疗前比较明显减少,差异有统计学意义(P<0.01);西药组与假手术组、模型组比较,差异有统计学意义(P<0.01),中药组与假手术组、模型组比较,差异有统计学意义(P<0.01),西药组与治疗组比较差异不明显,差异无统计学意义(P>0.05)。(2)VEGF阳性细胞数:与假手术组比较,模型组、西药组与中药组阳性细胞数增多,差异有统计学意义(P<0.05,P<0.01);与模型组比较,西药组和中药组阳性细胞数增多,差异有统计学意义(P<0.05);与西药组比较,中药组细胞数无明显增多,差异无统计学意义(P>0.05)。(3)SOD活性:与假手术组比较,模型组、西药组明显降低,差异有统计学意义(P<0.01),中药组降低不明显,差异无统计学意义(P>0.05);与模型组比较,西药组无明显变化,差异无统计学意义(P>0.05),中药组明显增高,差异�Objective To observe the effect of Huoling Yizhi Recipe on cognitive function,vascular endothelial growth factor(VEGF)expression and oxidative damage in hippocampus of vascular dementia(VD)rats.Methods 80 healthy male SPF SD rats were randomly divided into control group,sham operation group,model group and treatment group,with 20 rats in each group.The sham operation group and model group were given the same dose of normal saline,the control group was given nimodipine tablets,and the treatment group was given Huoling Yizhi Fang.Morris water maze test and shuttle box test were used in all four groups to detect the memory and space exploration ability of rats.Immunohistochemical method was used to detect VEGF expression in the brain of rats,and SOD activity and MDA content in the brain at the same time.Results(1)Morriss water labyrinth experiment:the model group,the western medicine group,and the traditional Chinese medicine group were significantly longer than the sham operation group before the use of the drug before the treatment(before treatment),and the difference was statistically significant(P<0.05).There was no significant changein the escape latency between the sham operation group and the model group before treatment and after medication(after treatment),and the difference was not statistically significant(P>0.05);the escape latency period after treatment in the Chinese medicine group and the Western medicine group was compared with that in this group Significantly reduced,the difference was statistically significant(P<0.01).Compared with the sham operation group and the model group,the difference between the western medicine group and the sham operation group was statistically significant(P<0.01).Compared with the sham operation group and the model group,the difference between the Chinese medicine group and the sham operation group and the model group was statistically significant(P<0.01).The difference was not obvious and had no statistical significance(P>0.05).(2)The number of VEGF positive cells:Compa

关 键 词：藿苓益智方 血管性痴呆 血管内皮生长因子 丙二醛 超氧化物歧化酶 

分 类 号：R285.5[医药卫生—中药学]