外源ATP对盐胁迫下油菜种子萌发及幼苗生长的影响  

Effects of exogenous ATP on seed germination and seedling growth of Brassica campestris under salt stress

作　　者：梁娟红 李巧丽 赖晶 张小花 张腾国[1] LIANG Juanhong;LI Qiaoli;LAI Jing;ZHANG Xiaohua;ZHANG tengguo(School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《干旱地区农业研究》2020年第3期89-96,共8页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金(31460099)。

摘　　要：以‘陇油8号’油菜为试验材料,研究了不同浓度(1、10、25、50、100μmol·L^-1)外源ATP预处理对盐胁迫下油菜种子萌发及幼苗生物量、H2O2和·OH含量、抗氧化酶活性、膜损伤程度、渗透调节物质及相关基因表达的影响。结果表明,与对照(不用ATP或NaCl处理)相比,用不同浓度的外源ATP处理,可不同程度地提高油菜种子的发芽率和发芽势,25μmol·L^-1的ATP处理表现较为显著,发芽率和发芽势分别提高了26.9%和17.2%。25μmol·L^-1的ATP+NaCl处理相比较于单独NaCl胁迫处理,油菜种子的发芽率和发芽势分别提高了13.7%和15.0%,油菜幼苗的生物量(根长、株高、鲜重)、2种抗氧化酶活性(CAT、POD)、可溶性糖和脯氨酸含量及相关基因(MAPK3和MAPK6、SOS1和NHX1)的表达量均显著增加,其中根长、株高、鲜重分别增加了23.6%、27.3%、28.6%,CAT、POD活性、可溶性糖和脯氨酸含量分别增加了10.3%、27.0%、15.9%、41.0%;而H2O2和·OH含量、丙二醛(MDA)含量及相对电导率分别显著降低了13.6%、6.3%、27.6%、20.5%。以上结果表明,外源ATP浸种能够促进NaCl胁迫处理下油菜的种子萌发,显著提高幼苗的生物量、抗氧化能力及相关基因的表达,降低其膜损伤,增强油菜幼苗的耐盐性。Using Brassica campestris“Longyou 8”as test material,effects of exogenous ATP pretreatment on the seed germination and seedling biomass,H2O2 and·OH content,antioxidant enzymes(CAT and POD)activities,membrane damage,osmoregulatory substances and expression of related genes(MAPK3 and MAPK6,SOS1 and NHX1)of Brassica campestris were studied.The results showed that compared with the control(without ATP or NaCl treatment),the germination rate and germination energy of Brassica campestris seeds were increased to different degrees with different concentrations(1,10,25,50μmol·L^-1 and 100μmol·L^-1)of exogenous ATP.The exogenous ATP treatment of 25μmol·L^-1 was particularly significant,and the germination rate and germination potential increased by 26.9%and 17.2%,respectively.Compared with NaCl stress treatment,25μmol·L^-1 exogenous ATP+NaCl treatment increased the germination rate and germination energy of Brassica campestris seeds by 13.7%and 15.0%,respectively;significantly increased the biomass(root length,plant height and fresh weight)of Brassica campestris seedling,two antioxidant enzyme activities(CAT、POD),soluble sugar and proline content and related gene(MAPK3 and MAPK6,SOS1 and NHX1)amount of express;increased root length,plant height,and fresh weight by 23.6%,27.3%and 28.6%,respectively.Also,the activities of CAT,POD,soluble sugar,and proline increased by 10.3%,27.0%,15.9%,and 41.0%,respectively,while the contents of H2O2 and·OH,malondialdehyde(MDA),and relative conductivity significantly decreased by 13.6%,6.3%,27.6%,and 20.5%,respectively.The results indicated that exogenous ATP soaking promoted the seed germination of Brassica campestris under NaCl stress,significantly increased the biomass,antioxidant capacity and expression of related salt-tolerant genes,reduced its membrane damage and enhanced the salt tolerance of Brassica campestris seedlings.

关 键 词：油菜 外源ATP浸种 NACL胁迫 种子萌发 抗逆生理 基因表达 

分 类 号：S634.3[农业科学—蔬菜学] Q945.7[农业科学—园艺学]