一种基于功能化修饰磁性纳米颗粒的石墨烯基光学生物传感器  

Graphene-Based Optical Biosensor Using Functionalized Magnetic Nanoparticles

作　　者：张慧琴 孙立勋 杨艺哲 吴晓静[2] 杨勇[1] 朱思伟[2] 袁小聪[3] Huiqin Zhang;Lixun Sun;Yizhe Yang;Xiaojing Wu;Yong Yang;Siwei Zhu;Xiaocong Yuan(Institute of Modern Optics,Nankai University,Tianjin 300071,China;Tianjin Union Medical Centre,Tianjin 300121,China;Nanophotonics Research Centre,Shenzhen Key Laboratory of Micro-Scale Optical Information Technology,Shenzhen University,Shenzhen,Guangdong 518060,China)

机构地区：[1]南开大学现代光学研究所,天津300071 [2]天津市人民医院,天津300121 [3]深圳大学深圳微尺度光信息技术重点实验室纳米光子学研究中心,广东深圳518060

出　　处：《光学学报》2020年第11期156-163,共8页Acta Optica Sinica

基　　金：国家自然科学基金(11774256);国家自然科学基金青年项目(61805157);天津市卫健委科技基金项目(2015KY30)。

摘　　要：提出了一种利用石墨烯基光学生物传感器对特异性生物小分子实现高灵敏检测的方法,采用易于修饰的磁性纳米颗粒作为生物探针载体,通过磁场控制实现磁性颗粒在石墨烯表面的吸附,优化了石墨烯表面复杂的改性过程。利用搭建的基于功能化修饰磁性纳米颗粒的石墨烯基光学生物传感器,实现了0.01 ng·mL^-1的赭曲毒素A的检测,在0.01~5 ng·mL^-1微小浓度范围内具有良好的响应,且表现出较好的特异性。该方法扩展了石墨烯基的光学生物传感器的应用范围,简化了生物分子的传感过程,提高了光学生物传感器的灵敏度。This study presents a high-sensitivity detection method for a specific biological molecule using a graphene-based optical biosensor.The use of functionalized magnetic nanoparticles as biological molecule carriers,which can be immobilized on the graphene surface under a magnetic field,improves the graphene surface-modification process.The effectiveness of our proposed method was successfully verified in an Ochratoxin A detection experiment.We obtained a detection limit of 0.01 ng·mL^-1 for Ochratoxin A,a good response in the range of 0.01--5 ng·mL^-1,and a good function of the biomolecule specificity.We believe that the proposed method can expand the scope of applications of graphene-based optical biosensors,simplify the biomolecule-sensing process,and improve the sensitivity of graphene-based optical biosensors.

关 键 词：生物光学 折射率传感 石墨烯 磁性纳米颗粒 生物毒素 

分 类 号：O436[机械工程—光学工程]