双抗原夹心法和间接法检测丙型肝炎病毒抗体的结果分析  

Analysis of the results of detection of hepatitis C virus antibody by double antigen sandwich method and indirect method

作　　者：黄爱琼[1] 肖韶英[1] 蓝岚茵[1] HUANG Ai-qiong;XIAO Shao-ying;LAN Lan-yin(Guangzhou Blood Center,Guangzhou Medical Key Laboratory,Guangzhou 510000,China)

机构地区：[1]广州血液中心,广州市医学重点实验室(血液安全重点实验室),510000

出　　处：《中国现代药物应用》2020年第14期244-246,共3页Chinese Journal of Modern Drug Application

基　　金：广州市医学重点学科建设项目(血液安全重点实验室)(项目编号:穗卫科教[2016]27号)。

摘　　要：目的比较双抗原夹心法和间接法检测丙型肝炎病毒抗体的结果。方法以血站进行无偿献血的人次作为研究对象,其中28982份标本使用双抗原夹心法检测作为实验组,27603份标本使用间接法检测作为对照组。比较两组的阳性检出率、复检率以及复检合格率,分析两种试剂的临床检测结果。结果实验组标本中检测出16份双试剂阳性标本,27份单试剂阳性可疑标本,对27份可疑标本进行复检后,有26份合格,1份不合格;对照组标本中检测出14份双试剂阳性标本,42份单试剂阳性可疑标本,对42份可疑标本进行复检后,有22份合格,20份不合格。实验组复检率为0.09%,复检合格率为96.30%;对照组复检率为0.15%,复检合格率为52.4%。实验组复检率低于对照组,复检合格率高于对照组,差异均具有统计学意义(P<0.05)。实验组双试剂阳性率为0.06%,对照组双试剂阳性率为0.05%,比较差异无统计学意义(P>0.05)。结论在血站对无偿献血人群进行检测时,可以选择双抗原夹心法对丙型肝炎病毒抗体进行检测,检测有效率要高于间接检测方法,降低了假阳性结果的出现几率,避免了血液浪费的现象出现,也减少了献血者淘汰的几率,值得在今后血站进行推广使用。Objective To compare the results of hepatitis C virus antibody detected by double antigen sandwich method and indirect method.Methods The number of unpaid blood donations at blood stations was taken as the study subject,of which 28982 specimens were tested by double antigen sandwich method as the experimental group,and 27603 specimens were tested by indirect method as the control group.The positive detection rate,retest rate and retest qualification rate were compared between the two groups,and the clinical test results of the two reagents was analyzed.Results In the experimental group,16 double-reagent positive specimens and 27 single-reagent positive suspicious specimens were detected.After retest of 27 suspicious specimens,26 specimens were qualified and 1 specimen failed.In the control group,14 double-reagent positive specimens and 42 single-reagent positive suspicious specimens were detected.After retest of 42 suspicious specimens,22 specimens were qualified and 20 specimens failed.The retest rate of the experimental group was 0.09%and retest qualification rate was 96.30%.The retest rate of the control group was 0.15%and retest qualification rate was 52.4%.The retest rate of the experimental group was lower than that of the control group,and retest qualification rate was higher than that of the control group,and the difference was statistically significant(P<0.05).The double reagent positive rate of the experimental group was 0.06%,which was 0.05%of the control group,and the difference was not statistically significant(P>0.05).Conclusion When testing unpaid blood donors in blood stations,double antigen sandwich method is good choice to detect hepatitis C virus antibody.The detection efficiency is higher than the indirect detection method,which reduces the probability of false positive results and avoids the waste of blood.The appearance also reduces the chance of blood donors being eliminated,and it is worth promoting and using in blood stations in the future.

关 键 词：双抗原夹心法 间接法 检测 丙型肝炎病毒抗体 结果分析 

分 类 号：R446.6[医药卫生—诊断学] R512.63[医药卫生—临床医学]