银杏黄酮抗脑缺血再灌注损伤的作用机制研究  

Mechanisms of ginkgetin against experimental cerebral ischemia/reperfusion injury

作　　者：骞秀芳 孙会仙 Qian Xiufang;Sun Huixian(The Hospital of Beijing Armed Police Forces,Beijing 100027)

机构地区：[1]武警北京市总队医院,北京100027

出　　处：《基层医学论坛》2020年第22期3128-3130,共3页The Medical Forum

摘　　要：目的探讨银杏黄酮预处理对实验性脑缺血再灌注损伤(cerebral ischemia/reperfusion injury,CI/RI)的作用机制。方法将50只沙鼠随机分为银杏黄酮组(200,100,50 mg/kg)、模型组、假手术组,模型制备前3 d各组开始腹腔注射给药,通过双侧颈总动脉夹闭术制备CI/RI模型,采用卒中指数评估再灌注6 h内的神经症状,采用免疫组化法检测再灌注6 h后脑海马组织细胞间黏附分子1(intercellular cell adhesion molecule-1,ICAM-1)的表达。结果与模型组卒中指数评分[(18.9±2.5)分]比较,银杏黄酮预处理CI/RI沙鼠高剂量[(12.9±2.2)分]、中剂量[(14.5±2.4)分]、低剂量[(14.9±3.6)分]均有显著降低,差异有统计学意义(P<0.01);与模型组海马CA1区ICAM-1的表达(41.58±8.26)相比,银杏黄酮各剂量预处理均可明显下调其阳性表达,高、中剂量组为(25.84±6.22)、(26.37±8.65),低剂量组表达为(32.03±9.10),差异均有统计学意义(P<0.05或P<0.01)。结论银杏黄酮预处理保护CI/RI沙鼠脑组织的作用机制可能与下调ICAM-1表达有关。Objective To explore the mechanisms of ginkgetin pretreatment on gerbils with cerebral ischemia/reperfusion injury(CI/RI).Methods 50 gerbils were randomly divided into ginkgetin high,medium and low dose groups(200,100 and 50 mg/kg,respectively),sham and model groups.Ginkgetin was intraperitoneally injected for 3 days before the model preparation.CI/RI model in gerbil was achieved by bilateral common carotid occlusion followed by 6-hour reperfusion.Stroke index was used to evaluate neurological symptoms of gerbils during 6-hour reperfusion.The intercellular cell adhesion molecule-1(ICAM-1)activities in hippocampi tissue of CI/RI gerbils were examined by immunohistochemistry after 6-hour reperfusion.Results Compared with stroke index(18.9±2.5)of model group,those in ginkgetin high,medium and low dose pretreatment groups were(12.9±2.2),(14.5±2.4)and(14.9±3.6),respectively,which were markedly improved(P<0.01).Compared with ICAM-1 expression(IOD:41.58±8.26)in hippocampi CA1 tissue of model group,those in ginkgetin high,medium and low dose pretreatment groups were(25.84±6.22),(26.37±8.65)and(32.03±9.10),respectively,which were significantly reduced(P<0.05 or P<0.01).Conclusion Ginkgetin pretreatment could protect the brain tissue of CI/RI gerbils,its action maybe related with the inhibition of ICAM-1 expression.

关 键 词：实验性脑缺血再灌注损伤 沙鼠 银杏黄酮 作用机制 细胞间黏附分子1 

分 类 号：R285.5[医药卫生—中药学]