截叶铁扫帚中芦丁、槲皮素与山奈酚含量测定研究  

Determination Research of Rutin,Quercetin and Kaemphenol in Lespedeza cuneata

作　　者：董洪强 赵跃丽 张兰胜 Dong Hongqiang;Zhao Yueli;Zhang Lansheng(School of Pharmacy and Chemistry, Dali University, Dali 671000, China;College of Applied Technology, Lijiang Teachers College, Lijiang 674199, China)

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]丽江师范高等专科学校应用技术学院,云南丽江674199

出　　处：《中国野生植物资源》2020年第7期18-21,共4页Chinese Wild Plant Resources

基　　金：云南省科技厅地方本科院校联合基金项目(2019FH001)。

摘　　要：目的:建立一种同时测定截叶铁扫帚中3个黄酮类成分(芦丁、槲皮素、山奈酚)含量的方法。方法:采用RP-HPLC法,ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),甲醇-0.4%磷酸为流动相,检测波长371 nm,流速为0.7 mL/min,柱温35℃。结果:芦丁在0.108~1.681μg(r=0.9993)有良好的线性关系,槲皮素在0.204~4.083μg(r=0.9996)有良好的线性关系,山奈酚在0.072~1.440μg(r=0.9993)有良好的线性关系;平均加样回收率分别为99.37%(RSD=1.70%)、100.10%(RSD=1.80%)、99.42%(RSD=0.79%)。结论:建立的方法重复性好,精密度高,稳定性好,有很好的分离效果和较高的分析准确度,可用于截叶铁扫帚药材的质量控制。Objective:To establish a method for the simultaneous determination of rutin,quercetin and kaemphenol in Lespedeza cuneata.Methods:The content of rutin,quercetin and kaemphenol was determined by reversed phase high performance liquid chromatography(RP-HPLC).The chromatographic column was ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm,5μm).The mobile phase was methanol-0.4%phosphoric acid.The detection wavelength was 371 nm.The flow rate was 0.7 mL/min and the column temperature was kept at 35℃.Results:The linear ranges for rutin,quercetin and kaemphenol were 0.108-1.681μg(r=0.9993),0.204-4.083μg(r=0.9996),0.072-1.440μg(r=0.9993).The average recoveries for three flavonoids were 99.37%(RSD=1.70%),100.10%(RSD=1.80%),99.42%(RSD=0.79%),respectively.Conclusion:The method has a good reproducibility,high precision,good stability,good separation effect and high analytical accuracy,and can be used for the quality control of L.cuneata.

关 键 词：截叶铁扫帚 芦丁 槲皮素 山奈酚 RP-HPLC法 

分 类 号：R284.1[医药卫生—中药学]