宽叶独行菜提取物对大鼠胃肠动力及SP和Ghrelin的影响  

Effects of Lepidium latifolium L. extracts on rats gastrointestinal motility,SP and Ghrelin

作　　者：赵乙桦 钟江斌 李威霖 祁得胜 孙士浩 邹登朗 都玉蓉 马建滨 ZHAO Yi-hua;ZHONG Jiang-bin;LI Wei-lin;QI De-sheng;SUN Shi-hao;ZOU Deng-lang;DU Yu-rong;MA Jian-bin(Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of Qinghai-Tibetan Plateau in Qinghai Province/of Medicinal Plant and Animal Resources of Qinghai-Tibetan Plateau in Qinghai Province,College of Life Sciences,Qinghai NormaKl UeyniLvaebrsoirtayt,ory Xining 810008,China)

机构地区：[1]青海师范大学生命科学学院青海省青藏高原生物资源多样性形成机制与综合利用重点实验室/青海省青藏高原药用动植物资源重点实验室,西宁810008

出　　处：《湖北农业科学》2020年第9期130-132,共3页Hubei Agricultural Sciences

基　　金：青海省基础研究计划项目(2019-ZJ-916);青海省重点研发与转化项目(2019-SF-124)。

摘　　要：为研究宽叶独行菜(Lepidium latifolium L.)提取物对胃肠动力障碍大鼠的影响,并初步探讨其作用机制,选取24只SPF级SD大鼠,体重180~200 g,随机分成空白组、模型组和受试药物组,每组8只,雌雄各半,各组大鼠灌胃给予相应的药物,受试药物组每只大鼠灌胃2 mL宽叶独行菜提取物溶液(0.36 g/mL),空白组和模型组每只大鼠灌胃等体积去离子水。每天1次,连续灌胃6 d后禁食不禁水24 h,第7天正常给药1 h后,模型组和受试药物组大鼠腹腔注射硫酸阿托品2 mg/kg,空白组大鼠腹腔注射等体积的生理盐水。20 min后,所有大鼠均灌胃0.4 mL 2%蓝色葡聚糖-2000溶液,30 min后腹腔注射4 mL/kg的10%水合氯醛溶液麻醉大鼠,取材测定胃排空率和小肠推进率,ELISA法测定胃和十二指肠匀浆中P物质(SP)和饥饿素(Ghrelin)浓度。结果表明,与空白组比较,模型组大鼠胃排空率和小肠推进率极显著降低(P<0.01),胃、十二指肠中SP浓度和胃中Ghrelin浓度无明显变化(P>0.05),十二指肠中Ghrelin浓度极显著降低(P<0.01)。与模型组比较,受试药物组大鼠胃排空率和小肠推进率均极显著升高(P<0.01),胃中SP浓度显著升高(P<0.05);胃中Ghrelin浓度和十二指肠中SP、Ghrelin浓度均极显著升高(P<0.01)。表明宽叶独行菜提取物能改善硫酸阿托品致胃肠动力障碍大鼠的胃肠动力,提高其胃和十二指肠中SP和Ghrelin浓度可能是其发挥促胃肠动力作用的机制之一。To study the effects of the Lepidiumlatifolium L. extracts on rats with gastrointestinal motility disorder and explore its mechanism,24 SPF SD rats weighing 180~200 g were randomly divided into blank group,model group and test drug group. Each group had8 rats and the ratio of male to female is 1∶1. Each group was given the corresponding drugs by intragastric administration. Each rat in the test drug group was intragastrically administered with 2 mL solution of the extract from Lepidiumlatifolium L.(0.36 g·mL^-1). Each rat in the blank group and the model group was intragastrically administrated with equal volume of distilled water. After intragastric administration once a day for 6 days,the rats were fasted for 24 h but free of water. on the 7 th day,1 h after the normal administration,the model group and the test group were intraperitoneally injected with atropine sulfate of 2 mg·kg^-1,and the blank group was intraperitoneally injected with the same volume of normal saline. After 20 min,all rats were intragastrically administered with 0.4 ml of 2%blue dextran-2000 solution. After 30 min,rats were anesthetized with 4 mL·kg^-1 of 10% chloral hydrate. The detect index included gastric emptying rate,small intestinal propulsion rate,the substance P(SP)and ghrelin concentrations in gastric and duodenal homogenate by ELISA. The results showed that the gastric emptying rate and small intestinal propulsion rate in the model group were extremely significant lower than those in blank group(P<0.01)while the gastric and duodenal SP concentrations and the gastric ghrelin concentration presented no significant difference(P>0.05),and the concentration of duodenal ghrelin was significantly higher(P<0.01). Compared with the model group,gastric emptying rate and small intestinal propulsion rate in the test drug group were also significantly increased(P<0.01),and the concentration of SP in gastric was significantly increased(P< 0.05);the concentration of ghrelin in gastric and the concentration of SP,ghrelin in duodenal were s

关 键 词：宽叶独行菜(Lepidium latifolium L.) 大鼠胃肠动力 P物质(SP) 饥饿素(Ghrelin) 

分 类 号：S567.239[农业科学—中草药栽培] R915[农业科学—作物学]