北虫草CNZ虫草酸提取方法的筛选和优化  

Screening and optimization of cordycepic acid extraction method of Cordyceps militaris CNZ

作　　者：林爱华[1] 苗莉云 王俊玲 李惠 宋发军[1] 张鹏[1] LIN Ai-hua;MIAO Li-yun;WANG Jun-ling;LI Hui;SONG Fa-jun;ZHANG Peng(National Committee Key Laboratory for Biotechnology/Hubei Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China/College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China;College of Basic Medical Sciences,Shanxi University of Chinese Medicine,Jinzhong 030619,Shanxi,China)

机构地区：[1]中南民族大学生命科学学院/生物技术国家民委重点实验室/武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉430074 [2]山西中医药大学基础医学院,山西晋中030619

出　　处：《湖北农业科学》2020年第9期141-144,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金(31370118,31770134);“武陵山区特色资源植物种质保护与利用湖北省重点实验室建设”基金项目(2018BFC360);中南民族大学基本科研业务费专项资金资助项目(CZT18005,18006,18007)。

摘　　要：为获得北虫草(Cordyceps militaris)CNZ虫草酸最适的提取方法,比较了6种虫草酸提取方法,结果表明超声辅助醇提法虫草酸的得率最高,达113.31 mg/g,可作为初始提取方法。进一步通过单因素试验和正交试验优化提取次数、固液比、超声时间、乙醇浓度等条件,获得北虫草CNZ虫草酸提取条件为:乙醇浓度20%(体积分数),固液比1∶10(g∶mL),超声时间20 min,提取次数3次;在优化的提取条件下,北虫草CNZ虫草酸得率为161.06 mg/g,与初始提取条件相比提高了42.1%。In order to obtain the optimal cordycepic acid extraction method of Cordyceps militaris CNZ,six extraction methods were used to extract cordycepic acid of C. militaris CNZ,respectively,and the extraction yield of cordycepic acid with ultrasonicassociated ethanol extraction method was the maximum for 113.31 mg/g,so was selected as one suitably initial method. Subsequently,the extraction conditions,including extraction times,solid-liquid ratio,ultrasonic time and ethanol concentration,were further optimized by single factor experiments and orthogonal experiments. One optimized cordycepic acid extraction method of C. militaris CNZ was obtained as that:ethanol concentration was 20%(volume fraction),solid-liquid ratio was 1∶10(g∶mL),ultrasonic time was 20 min and extraction times was three. Using the optimized extraction method,the extraction yield of cordycepic acid of C. militaris CNZ was 161.06 mg/g,which was about 42.1% higher than that using the initial extraction method.

关 键 词：北虫草(Cordyceps militaris) 虫草酸 提取方法 优化 

分 类 号：S567.39[农业科学—中草药栽培]