农杆菌介导的冷诱导基因水稻遗传转化体系的建立  

Establishment of Genetic Transformation System of Cold-induced Gene Mediated by Agrobacterium in Rice

作　　者：崔永祯[1] 赵红[1] 黄格 郑爱清[1] 李社萍[1] 李鸣[2] CUI Yong-zhen;ZHAO Hong;HUANG Ge;ZHENG Ai-qing;LI She-ping;LI Ming(Institute of Food Crops,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530005,China)

机构地区：[1]云南省农业科学院粮食作物研究所,云南昆明650205 [2]广西农业科学院甘蔗研究所,广西南宁530005

出　　处：《江西农业学报》2020年第7期6-11,共6页Acta Agriculturae Jiangxi

基　　金：广西科学研究与技术开发计划项目(桂科能1347012-1)。

摘　　要：利用农杆菌介导技术,以Bar基因为筛选标记,用高山离子芥的冷诱导基因Cbcor15a侵染水稻品种日本晴的愈伤组织,经分化诱导后获得了再生植株。经Cef和PPT筛选,共获得转化Cbcor15a基因的日本晴再生苗9株;经PCR检测和Basta涂抹实验,结果证明有6株再生苗呈阳性。本遗传转化体系的建立可为利用转Cbcor15a基因选育具有耐冷性的水稻新品种提供参考。The Bar gene was used as screening marker in the study,and the cold induced gene Cbcor15a from Chorispora bungeana was used as the target gene to transferr into rice callus by Agrobacterium-mediated method.Regenerated plants were obtained after differentiation.Through Cef and PPT screening,nine Chorispora bungeana Cbcor15a tranformed plants were got.PCR detection and Baste test proved that six of the plants were positive.The establishment of the genetic transformation system could provide reference for the breeding of new rice varieties with cold tolerance by Cbcor15a gene.

关 键 词：水稻 农杆菌介导法 冷诱导基因 遗传转化体系 建立 

分 类 号：S511.032[农业科学—作物学]