恩施巴戟环烯醚萜苷类成分提取工艺优选及抗氧化活性研究  

Study on extraction process and antioxidant activity of iridoid glycosides in Damnacanthus officinarum Huang

作　　者：段金辉 刘阳[1,2] 黄容吉 胡杨 阮金兰 DUAN Jinhui;LIU Yang;HUANG Rongji;HU Yang;RUAN Jinlan(School of Life Science,Wuchang University of Technology,Wuhan 430223,China;School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China)

机构地区：[1]武昌理工学院生命科学学院,湖北武汉430223 [2]武汉工程大学环境生态与生物工程学院,湖北武汉430205

出　　处：《药学实践杂志》2020年第4期346-349,共4页Journal of Pharmaceutical Practice

摘　　要：目的优化恩施巴戟中主要环烯醚萜苷类成分提取工艺,评价恩施巴戟体外抗氧化活性。方法选择经典的加热回流提取法,以乙醇体积分数、溶剂倍量、提取时间为考察因素,以浸膏得率和水晶兰苷含量的综合评分为评判指标,设计正交试验筛选最佳提取工艺条件;测定总还原能力、DPPH清除率、羟自由基清除率,检测其体外抗氧化活性。结果最佳提取工艺条件为6倍量的60%的乙醇加热回流2 h;恩施巴戟具有一定的抗氧化能力,乙酸乙酯部位活性最佳。结论优化的提取工艺稳定可行,可用于提取恩施巴戟的环烯醚萜苷类成分,该研究初步证明了恩施巴戟具有一定的抗氧化活性。Objective To optimize the extraction process of main iridoid glycosides in Damnacanthus officinarum Huang and evaluate the antioxidant activity of Damnacanthus officinarum Huang in vitro. Methods The classical heating-reflux extraction method was selected. The volume fraction of ethanol, the volume of solvent and extraction time were taken as the evaluation factors. The comprehensive score of extraction yield and the monotropein content were used as the evaluation indexes.An orthogonal test was designed to select the best extraction conditions. The total reducing capacity, DPPH clearance rate and hydroxyl radical scavenging rate were measured to determine its antioxidant activity in vitro. Results The optimal extraction process was the reflux with 6 times volume of 60% ethanol for 2 hours. Damnacanthus officinarum Huang has certain antioxidant capacity, and the activity of ethyl acetate part had the best effect. Conclusion The optimized extraction process is stable and feasible, which can be used for extraction of the iridoid glycosides from Damnacanthus officinarum Huang. This study has proved that Damnacanthus officinarum Huang has certain antioxidant activity.

关 键 词：恩施巴戟 水晶兰苷 正交试验 高效液相色谱法 抗氧化 

分 类 号：R284.2[医药卫生—中药学]