GLP-1受体对脂多糖诱导的肾小管上皮细胞焦亡的影响  

Effect of GLP-1 receptor on the lipopolysaccharide induced pyroptosis of renal tubular epithelial cells

作　　者：艾晨牧 段智[1] 李桂成[1] 雷小保[1] Ai Chenmu;Duan Zhi;Li Guicheng;Lei Xiaobao(Department of Critical Care Medicine,The First People’s Hospital of Chenzhou,Chenzhou 423000,China)

机构地区：[1]郴州市第一人民医院重症医学科,423000

出　　处：《国际医药卫生导报》2020年第13期1832-1835,共4页International Medicine and Health Guidance News

基　　金：湖南省自然科学基金(2018JJ3015,2018JJ6004);湘南学院科研课题(2019XJ80);郴州市科技发展计划项目(zdyf201924);郴州市第一人民医院课题(N2019-057)。

摘　　要：目的探讨GLP-1受体对脂多糖诱导的肾小管上皮细胞焦亡的影响。方法NRK-52E细胞随机分为4组:对照组细胞不接受任何刺激;模型组细胞接受10μg/ml脂多糖(LPS)刺激12 h;低剂量治疗组细胞接受10μg/ml LPS及50 nmol/L利拉鲁肽刺激12 h;高剂量治疗组细胞接受10μg/ml LPS及100 nmol/L利拉鲁肽刺激12 h。采用乳酸脱氢酶法检测细胞毒性;CCK-8试剂盒检测细胞活力;试剂盒检测caspase-1活性;ELISA试剂盒检测细胞培养液IL-1β及IL-18水平。结果与对照组比较,模型组细胞毒性、caspase-1活性、培养液IL-1β及IL-18水平分别显著增加为(302.0±21.5)U/L、(376.0±35.2)%、(423.0±43.2)pg/ml及(285.0±25.7)pg/ml,细胞活力显著下降为(76.0±4.2)%;与模型组比较,低剂量治疗组细胞毒性、caspase-1活性、培养液IL-1β及IL-18水平均无显著变化;与模型组比较,高剂量治疗组细胞毒性、细胞活力、caspase-1活性、培养液IL-1β及IL-18水平分别显著下降为(163.0±14.6)U/L、(228.0±25.0)%、(272.0±25.8)pg/ml及(154.0±14.7)pg/ml,细胞活力显著增加为(88.0±5.0)%。结论激活GLP-1R可以显著抑制LPS诱导的肾小管上皮细胞焦亡,促进细胞存活。Objective To investigate the effects of glucagon like peptide-1(GLP-1)receptor on the lipopolysaccharide induced pyroptosis of renal tubular epithelial cells.Methods NRK-52E cells were randomly divided into four groups:cells in the control group received none treatment;cells in the model group received the treatment of LPS(10μg/ml)for 12 hours;cells in the low-dose treatment group received the treatment of LPS(10μg/ml)and liraglutide(50 nmol/L)for 12 hours;cells in the high-dose treatment group received the treatment of LPS(10μg/ml)and liraglutide(100 nmol/L)for 12 hours.The cytotoxicity was measured by the LDH assay kit;the cell viability was measured by the CCK-8 kit;the caspase-1 activity was measured by the caspase-1 activity assay kit;the concentrations of IL-1βand IL-18 in the culture medium were determined by ELISA kits.Results Compared with the control group,the cytotoxicity,caspase-1 activity,the concentrations of IL-1βand IL-18 in the culture medium in the model group respectively increased to(302.0±21.5)U/L,(376.0±35.2)%,(423.0±43.2)pg/ml,and(285.0±25.7)pg/ml,the cell viability decreased to(76.0±4.2)%.Compared with the model group,the cell viability,cytotoxicity,caspase-1 activity,the concentrations of IL-1βand IL-18 in the culture medium in the low-dose treatment group had not significantly changed.Compared with the model group,the cytotoxicity,caspase-1 activity,the concentrations of IL-1βand IL-18 in the culture medium in the high-dose treatment group respectively decreased to(163.0±14.6)U/L,(228.0±25.0)%,(272.0±25.8)pg/ml,and(154.0±14.7)pg/ml,the cell viability increased to(88.0±5.0)%.Conclusion Activation of GLP-1R significantly alleviates LPS-induced pyroptosis,and promotes cell survival.

关 键 词：GLP-1受体 脓毒症 急性肾损伤 细胞焦亡 

分 类 号：R692[医药卫生—泌尿科学]