连续单柱多维液相色谱法制备超高纯度的草乌甲素  

Preparation with Ultra-high Purity of Bulleyaconitine A by On-line Multidimensional Liquid Chromatographic System with a Single Column

作　　者：宁晓辉 欧阳鹏 耿信笃 王改改 Ning Xiaohui;Ouyang Peng;Geng Xindu;Wang Gaigai(Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,Institute of Modern Separation Science,Shaanxi Provincial Key Laboratory,Northwest University,Xi’an 710069;School of Information&Technology,Northwest University,Xi’an 710069;SUZHOU PRIMAS Inc.,Suzhou 215000,China)

机构地区：[1]西北大学化学与材料科学学院,陕西西安710069 [2]西北大学信息学院,陕西西安710069 [3]苏州普瑞马斯科技有限公司,江苏苏州215000

出　　处：《广东化工》2020年第14期248-249,共2页Guangdong Chemical Industry

摘　　要：多维高效液相色谱快速纯化物质是当前色谱领域一个重要的发展方向。本实验首次对全自动多维高效液相色谱系统进行了优化装配,并对草乌甲素(BLA)进行连续单柱三维的分离。从实验结果看出,本实验采用的在线单柱多维分离系统比普通的离线三维色谱分离纯化要快得多;因其在线的密闭系统实施全部操作,样品质量回收率更高。Multidimensional high performance liquid chromatography(MD-LC) system is a hot-point of research in the field of liquid chromatography. In this experiment, we used a fully-automatic MD-LC system firstly to separate and purify bulleyaconitine A(BLA) of ultra-high purity by 3 D-LC with a single reverse phase liquid chromatographic(RPLC) column. In comparing with conventional 3 D-LC by means of off-line manner, the online 3 D-LC system with the single RPLC column has some advantages of high speed, high mass recovery, due to all operations to be carried out in a closed system.

关 键 词：草乌甲素 多维液相色谱 反相液相色谱 纯度 

分 类 号：O657.72[理学—分析化学]