华东地区猪繁殖与呼吸综合征流行病学调查研究  

Epidemiological Survey of Porcine Reproductive and Respiratory Syndrome in East China

作　　者：洪伟鸣[1] 左伟勇[1] 宋亮[1] 吉光杰 张晔 杨佳文 陈海峰[1] HONG Wei-ming;ZUO Wei-yong;SONG Liang(Jiangsu Animal Husbandry&Verterinary College,Taizhou,Jiangsu 225300)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《安徽农业科学》2020年第14期92-93,96,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省2020年大学生创新创业训练计划项目;江苏省2017年高校优秀科技创新团队项目。

摘　　要：为了解华东地区猪繁殖与呼吸综合征的感染状况,应用逆转录-聚合酶链反应(RT-PCR)/聚合酶链反应(PCR)方法对2017—2018年在华东地区各大中小规模猪场采集的210份临床样品进行病原学检测(肺、脾、淋巴结等),并对分离的猪繁殖与呼吸综合征病毒(PRRSV)进行测序,由于2017—2018年江苏、安徽和福建一些中小养殖场发生小规模的流产死胎,因此也对其他一些相关常见病毒[如猪圆环病毒Ⅱ型(PCV2)、猪瘟病毒(CSFV)、猪伪狂犬病毒(PRV)]进行了检测,以确定导致流产死胎的主要原因以及猪繁殖与呼吸综合征的流行情况和流行毒株。结果表明:210份样品中,PRRSV阳性样品78份,阳性率为37.1%;PCV2阳性样品189份,阳性率90.0%;PRV阳性样品5份,阳性率2.4%;CSFV阳性样品0份,阳性率0。在这些阳性样品中,福建、浙江、上海、江苏、安徽、江西和山东等地区抗原阳性率有所差别,但都以PRRSV和PCV2为主。通过此次调查了解了华东地区猪繁殖与呼吸综合征的基本流行情况,为今后猪繁殖与呼吸综合征的防控提供了参考。In order to obtain the infection status of porcine reproductive and respiratory syndrome in East China,we used RT-PCR/PCR method to detect pathogens in 210 clinical samples collected from large and medium-sized pig farms in East China during 2017-2018(lung,spleen,lymph nodes,etc.,and sequenced the isolated PRRSV.Due to small-scale abortions in some small and medium-sized farms in Jiangsu,Anhui and Fujian during 2017-2018,we also have other related common viruses such as porcine circovirus type 2(PCV2),classical swine fever virus(CSFV),and pseudorabies virus(PRV)were tested to determine the main causes of stillbirth in abortion and the prevalence and prevalence of porcine reproductive and respiratory syndrome.The results showed that among 210 samples,there were 78 positive samples with the positive rate of 37.1%;189 PCV2 positive samples with the positive rate of 90.0%;5 positive PRV samples with the positive rate of 2.4%;and CSFV positive sample was 0,the positive rate was 0.Among these positive samples,the positive rates of antigens in Fujian,Zhejiang,Shanghai,Jiangsu,Anhui,Jiangxi and Shandong were different,but they were mainly PRRSV and PCV2.Through this survey,we learned the basic epidemic situation of blue ear disease in East China,and provided references for the prevention and control of porcine reproductive and respiratory syndrome in pigs.

关 键 词：猪繁殖与呼吸综合征 流行病学 阳性率 

分 类 号：S851.3[农业科学—预防兽医学]