花椰菜黑腐病苗期抗性鉴定方法研究  

Study on Resistance Identification Method of Black Rot at Seedling Stage of Cauliflower

作　　者：姚玉荣 霍建飞 郝永娟 贲海燕 王万立 YAO Yu-rong;HUO Jian-fei;HAO Yong-juan(Tianjin Kernel Vegetable Research Institute/State Key Laboratory of Vegetable Germplasm Resources Innovation,Tianjin 300384;Tianjin Plant Protection Institute,Tianjin 300384)

机构地区：[1]天津科润农业科技股份有限公司/蔬菜种质创新国家重点实验室,天津300384 [2]天津市植物保护研究所,天津300384

出　　处：《安徽农业科学》2020年第14期137-139,共3页Journal of Anhui Agricultural Sciences

基　　金：蔬菜种质创新国家重点实验室开放课题(201802)。

摘　　要：为建立快速、高效的花椰菜黑腐病苗期抗性鉴定技术,以黑腐病病原Xanthomonas campestris pv.campestiss为菌源,高感黑腐病的花椰菜品种Y1-2为试验材料,研究了接种菌液浓度、苗龄、接种方法以及发病温度等因素对抗病性鉴定的影响,建立了优化的花椰菜黑腐病人工接种方法。结果表明,抗病性接种适宜条件为接种菌液浓度为1×108CFU/mL,接种时期为4~5叶期,喷雾法接种,发病温度28℃。该方法鉴定结果能够客观反映花椰菜品种的抗病性。In order to develop a rapid and efficient technique for evaluation of resistance to black rot by using artificial inoculation with the pathogenic Xanthomonas campestris pv.campestiss and the cauliflower variety Y1-2,the effects of inoculation concentration,seedling age,inoculation method and temperature were studied.The optimizing artificial inoculation methods for black rot identification were set up.The results showed that the key procedure was praying inoculum liquor of 1×108 CFU/mL on seedling with 4-5 true leaves stage keeping at 28℃.The identification results of the established assessment system can objectively and effectively reflect the resistance of cauliflower.

关 键 词：花椰菜 黑腐病 抗性鉴定 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]