HPLC法同时测定双黄连制剂中9种成分的含量  

Simultaneous determination of 9 components in Shuanghuanglian preparations by HPLC

作　　者：陈蓉 陈伟[2] 王思渊 郝刚 CHEN Rong;CHEN Wei;WANG Siyuan;HAO Gang(Suzhou Institute for Drug Control,Suzhou 215104,China;Suzhou Hospital of Traditonal Chinese Medicine,Suzhou 215002,China)

机构地区：[1]苏州市药品检验检测研究中心,苏州215104 [2]苏州市中医医院,苏州215002

出　　处：《西北药学杂志》2020年第4期517-522,共6页Northwest Pharmaceutical Journal

基　　金：苏州市科技计划项目(编号:SYS201781)。

摘　　要：目的建立能同时测定4种双黄连常用制剂中黄芩苷、咖啡酸、连翘苷、3,5-O-二咖啡酰基奎宁酸、汉黄芩素、绿原酸、木犀草素、黄芩素和木犀草苷9种成分含量的HPLC法。方法采用Sepax Amethyst C18-H色谱柱,流动相为乙腈(A)-1 mL·L^-1磷酸溶液(B),梯度洗脱;流速:1 mL·min^-1;检测波长:280 nm;柱温:35℃。结果 9种成分均在40 min内出峰,分离度符合要求,线性关系良好,回收率均在95.96%~97.92%(n=6)范围内,RSD值均小于3.0%。结论该方法方便、易行、实用性强,可用于不同双黄连制剂的质量控制。Objective To establish HPLC chromatographic method for the determination of baicalin,caffeic acid,phiiiyrin,3,5-O-dicarboxylquinic acid,wogonin,chlorogenic acid,luteolin,baicalein and luteoside in 4 commonly used Shuanghuanglian preparations.Methods Separation was performed on a Sepax Amethyst C18-H column.The mobile phase consisted of acetonitrile(A) and 1 mL·L^-1 phosphoric acid solution(B) with a gradient elution.The flow rate was 1 mL·min^-1.The detection wavelength was set at 280 nm and the column temperature was maintained at 35 ℃.Results The 9 components were separated within 40 min and the separation degree conformed to the requirement.The linear relationships were good.The recovery rates were between 95.96% and 97.92%(n=6),and the RSDs were all less than 3.0%.Conclusion The method is convenient and easy,and has good practicability.It can be used for the quality control of different preparations of Shuanghuanglian.

关 键 词：黄芩苷 双黄连制剂 含量测定 HPLC法 

分 类 号：R927.2[医药卫生—药学]