四氯化碳造模方法诱导下肝纤维化形成对大鼠结肠组织的影响  

Efficacy of liver fibrosis induced by carbon tetrachloride modeling on colonic tissue in rats

作　　者：刘容 赵壮志[1] 刘旭东[1] 吕萍[1] 徐新杰[1] 赵晓芳[1] Liu Rong

机构地区：[1]广西中医药大学附属瑞康医院,广西南宁530000

出　　处：《中医临床研究》2020年第16期6-9,共4页Clinical Journal Of Chinese Medicine

基　　金：国家自然科学基金(No.81473532);广西一流学科建设重点课题(No.2018XK084);广西中医药管理局课题(GZLC14-10);广西自然科学基金青年基金项目(No.2018GXNSFBA050041);广西卫计委自筹课题(Z2016205)。

摘　　要：目的:探讨四氯化碳(Carbon Tetrachloride,CCl4)肝纤维化的造模方法对大鼠结肠组织的影响。方法:采用清洁级雄性Wistar大鼠,设立正常组和模型组,采用CCl4花生油混合液皮下注射大鼠构建肝纤维化模型,常规苏木精-伊红染色法(Hematoxylin-eosin Staining,HE)染色,通过扫描电镜检测CCl4大鼠肝纤维化模型中结肠组织的病理变化。结果:成功复制肝纤维化大鼠模型。肝纤维化模型组中结肠组织HE染色显示,大鼠结肠组织中细胞结构较清晰,肠黏膜绒毛结构较完整,无明显肠绒脱落现象,上皮细胞排列较为整齐,杯状细胞能够较好保存完整,细胞外壁能够保存完整,组织间无明显充血、肿胀等表现,与正常组相比,结肠组织无明显变化;扫描电镜显示,肝纤维化模型组结肠组织上皮细胞未见破损,也未见明显肿胀,可见杯状细胞,上皮细胞表面微绒毛排列整齐,未见脱落,细胞间隙未见增宽,细胞也未见萎缩,与正常组相比,结肠组织无明显变化。结论:CCl4造模方法诱导下大鼠肝纤维化形成可能对结肠组织无影响。Objective:To investigate the effect of liver fibrosis modeling induced by CCl4 on colonic tissue in rats.Methods:The clean-grade male Wistar rats were used to establish the normal group and the model group.The liver fibrosis model was constructed by subcutaneous injection of CCl4 peanut oil mixture,and the hematoxylin-eosin staining was performed by scanning electron microscopy.Pathological changes of colon tissue in a rat model of liver fibrosis induced by carbon tetrachloride were examined.Results:Model rats of liver fibrosis were successfully molded.In the liver fibrosis model group,the colonic tissue was stained by HE staining.The cell structure in the colonic tissue of rats was clear,the villus structure of intestinal mucosa was relatively complete,and there was no obvious shedding of intestinal velvet,and the epithe-lial cells were arranged neatly.The goblet cells were well preserved,the outer walls of the cells were preserved intact,there were no obvious hyp-eremia and swelling between tissues,and there was no obvious change in colon tissue compared with the normal group.In the liver fibrosis model group,the colonic epithelial cells were not damaged or swollen,goblet cells were seen,the microvilli on the surface of the epithelial cells were arranged neatly,no shedding,no widening of the cell space,no atrophy of the cells.There was no significant change in colonic tissue compared with the normal group.Conclusion:The formation of liver fibrosis induced by CCl4 modeling method may have no effect on colon tissue.

关 键 词：四氯化碳 结肠组织 肝纤维化造模方法 动物模型 

分 类 号：R656.9[医药卫生—外科学]